Rohde H, Vargas L, Hahn E, Kalbfleisch H, Bruguera M, Timpl R
Eur J Clin Invest. 1979 Dec;9(6):451-9. doi: 10.1111/j.1365-2362.1979.tb00912.x.
A sensitive and specific radioimmunoassay was developed for the precursor-specific peptide segment located at the amino end of bovine type III procollagen. Human material showed high cross-reactivity in this assay. Two forms of human procollagen peptides were detected in body fluids. The larger peptide (45K) was found in serum and ascites, and resembled the whole precursor-specific segment which is presumably released from human type III procollagen by a single enzymatic cleavage. The smaller peptide (10K) was found mainly in urine indicating that further degradation of circulating procollagen peptides is required prior to their passage through the kidney. Compared to peptide concentrations in normal human serum two to twenty-fold increases were observed in all patients with alcoholic liver disease, in fifteen of seventeen patients with acute hepatitis, and in ten of fourteen patients with chronic active hepatitis. Much higher levels were detected in ascites fluid. Patients with rheumatoid arthritis and other diseases showed far smaller elevations of the serum peptide. In alcoholic liver disease peptide levels correlated well with inflammation and necrosis observed in liver biopsies, but not with other laboratory parameters.
已开发出一种灵敏且特异的放射免疫分析法，用于检测位于牛III型前胶原氨基末端的前体特异性肽段。在该分析中，人体材料显示出高交叉反应性。在体液中检测到两种形式的人原胶原肽。较大的肽（45K）存在于血清和腹水中，类似于可能通过单次酶切从人III型前胶原释放的整个前体特异性片段。较小的肽（10K）主要存在于尿液中，表明循环中的原胶原肽在通过肾脏之前需要进一步降解。与正常人血清中的肽浓度相比，所有酒精性肝病患者、17例急性肝炎患者中的15例以及14例慢性活动性肝炎患者中的10例，肽浓度均升高了2至20倍。在腹水中检测到的水平要高得多。类风湿性关节炎和其他疾病患者的血清肽升高幅度要小得多。在酒精性肝病中，肽水平与肝活检中观察到的炎症和坏死密切相关，但与其他实验室参数无关。
