Greiner J V, Lass J H, Glonek T
Arch Ophthalmol. 1984 Nov;102(11):1676-7. doi: 10.1001/archopht.1984.01040031360027.
The concentrations of corneal phosphatic metabolites in fresh and eye-bank-processed corneas were measured by phosphorus 31 nuclear magnetic resonance of perchloric acid corneal extracts to determine whether metabolic differences exist between these two corneal preparations. Cat corneas were prepared using an eye-bank protocol, including specular microscopic examination and transport to a distant location. Fresh cat corneas were used as controls. The hexose 6-phosphate resonance band, the nucleoside monophosphate band, the phosphodiesters, and adenosine triphosphate of eye-bank-processed corneas were significantly decreased relative to fresh control corneas. Phosphatic metabolites that significantly increased relative to control corneas included inorganic orthophosphate and phosphocreatine. A calculated corneal tissue-energy index was significantly decreased for eye-bank-processed corneas relative to control. This decline demonstrates a compromise in the metabolic energy status of the tissue and is indicative of a diminished ability of the cornea to maintain its complement of high-energy phosphates.
通过对高氯酸角膜提取物进行磷31核磁共振，测量新鲜角膜和眼库处理过的角膜中角膜磷酸代谢物的浓度，以确定这两种角膜制剂之间是否存在代谢差异。使用眼库方案制备猫角膜，包括镜面显微镜检查并转运至远处。新鲜猫角膜用作对照。与新鲜对照角膜相比，眼库处理过的角膜的6-磷酸己糖共振带、核苷单磷酸带、磷酸二酯和三磷酸腺苷显著降低。相对于对照角膜显著增加的磷酸代谢物包括无机正磷酸盐和磷酸肌酸。相对于对照，眼库处理过的角膜的计算角膜组织能量指数显著降低。这种下降表明组织代谢能量状态受损，表明角膜维持其高能磷酸盐补充的能力减弱。
