Greiner J V, Lass J H, Glonek T
Arch Ophthalmol. 1984 Aug;102(8):1171-3. doi: 10.1001/archopht.1984.01040030949021.
Application of nondestructive and noninvasive nuclear magnetic resonance (NMR) techniques to the evaluation of corneal donor tissue metabolism may provide information that would allow improved selection of donor material for keratoplasty. A phosphorus 31 NMR spectrum was generated from a single intact human cornea, evaluating phosphatic metabolites qualitatively and quantitatively. The NMR analyses were conducted on four corneas (from patients aged from 73 to 75 years). Intracorneal pH (7.2) was monitored from the resonance shift position of inorganic orthophosphate (Pi). A numerical index (1.15) of tissue energy status was determined by comparing the relative tissue content of high-field, high-energy phosphate signals (adenosine triphosphate, adenosine diphosphate, nicotinamide-adenine dinucleotide, and the uridine diphospho-hexoses) to that of the low-field, low-energy phosphate signals (Pi, sugar phosphates, and phosphodiesters). This is the first demonstration of the feasibility of using nondestructive 31P NMR to monitor the metabolic status of a single intact eye bank cornea as would be required in the evaluation of eye bank tissue before keratoplastic procedures.
将无损和非侵入性核磁共振（NMR）技术应用于角膜供体组织代谢评估，可能会提供一些信息，从而有助于改进角膜移植供体材料的选择。从单个完整的人角膜获取了磷31 NMR光谱，对磷酸代谢物进行了定性和定量评估。对四个角膜（来自73至75岁的患者）进行了NMR分析。通过无机正磷酸盐（Pi）的共振位移位置监测角膜内pH值（7.2）。通过比较高场、高能磷酸盐信号（三磷酸腺苷、二磷酸腺苷、烟酰胺腺嘌呤二核苷酸和尿苷二磷酸己糖）与低场、低能磷酸盐信号（Pi、糖磷酸盐和磷酸二酯）的相对组织含量，确定了组织能量状态的数值指标（1.15）。这首次证明了使用无损31P NMR监测单个完整眼库角膜代谢状态的可行性，这是角膜移植手术前评估眼库组织所必需的。
