在假单胞菌属CBS 3的4-氯苯甲酸脱卤酶反应中，[18O]水掺入4-羟基苯甲酸中。
Incorporation of [18O]water into 4-hydroxybenzoic acid in the reaction of 4-chlorobenzoate dehalogenase from pseudomonas spec. CBS 3.
作者信息
Müller R, Thiele J, Klages U, Lingens F
出版信息
Biochem Biophys Res Commun. 1984 Oct 15;124(1):178-82. doi: 10.1016/0006-291x(84)90933-1.
PMID:6497878
Abstract
4-Chlorobenzoate is dehalogenated by 4-chlorobenzoate dehalogenase from Pseudomonas spec. CBS 3 to form 4-hydroxybenzoate. In 18O enriched water the hydroxygroup of 4-hydroxybenzoate is quantitatively labelled with 18O. This result clearly shows, that 4-chlorobenzoate dehalogenase catalyzes the hydrolytic cleavage of the halogen-carbon bond, without the involvement of molecular oxygen, a reaction not yet described.
摘要
来自假单胞菌属CBS 3的4-氯苯甲酸脱卤酶可使4-氯苯甲酸脱卤，生成4-羟基苯甲酸。在富含18O的水中，4-羟基苯甲酸的羟基被18O定量标记。这一结果清楚地表明，4-氯苯甲酸脱卤酶催化卤代碳键的水解断裂，且不涉及分子氧，这是一个尚未被描述的反应。
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