Department of Botany and Microbiology, The University of Oklahoma, 770 Van Vleet Oval, Norman, Oklahoma 73019.
Appl Environ Microbiol. 1990 Sep;56(9):2630-7. doi: 10.1128/aem.56.9.2630-2637.1990.
When di-, tri-, and tetrachloroaniline were incubated in methanogenic groundwater slurries, they were reductively dehalogenated by the aquifer microbiota. 2,3,4-Trichloroaniline was metabolized by two pathways. Primary dehalogenation occurred at either the meta or ortho position of this substrate to form 2,4- and 3,4-dichloroaniline, respectively. The latter chemical could be stoichiometrically converted to 3-chloroaniline. 2,3,4,5-Tetrachloroaniline was degraded by the sequential removal of halogens from the para and then the ortho position to form 3,5-dichloroaniline. An additional pathway was observed with this substrate when the aquifer slurries were amended with butyrate. That is, halogens could be removed from both the meta and ortho positions of tetrachloroaniline. The amendment of sulfate to methanogenic aquifer slurries slowed the rate of 2,3,4,5-tetrachloroaniline degradation and increased the amount of substrate channeled through the additional pathway. The reported intermediates or end products are identified by their chromatographic mobility and mass-spectral profiles.
当二氯、三氯和四氯苯胺在产甲烷地下水悬浮液中被孵育时，它们被含水层微生物群还原脱卤。2,3,4-三氯苯胺通过两种途径被代谢。主脱卤作用分别在该底物的间位或邻位发生，形成 2,4-和 3,4-二氯苯胺。后一种化学物质可以化学计量地转化为 3-氯苯胺。2,3,4,5-四氯苯胺通过从对位和邻位依次去除卤素而降解，形成 3,5-二氯苯胺。当含水层悬浮液中添加丁酸盐时，观察到该底物的另一种途径。也就是说，卤素可以从四氯苯胺的间位和邻位去除。向产甲烷含水层悬浮液中添加硫酸盐会降低 2,3,4,5-四氯苯胺的降解速度，并增加通过附加途径输送的底物量。报告的中间体或终产物通过其色谱迁移率和质谱图谱进行鉴定。
