来自正常和致瘤性人类细胞的DIDS结合蛋白表达的比较。
A comparison of the expression of the DIDS-binding proteins from normal and tumorigenic human cells.
作者信息
Banyard M R
出版信息
Br J Cancer. 1984 Dec;50(6):809-14. doi: 10.1038/bjc.1984.260.
Abstract
A monoclonal antibody was prepared against DIDS, an inhibitor of anion transport, and used to compare the occurrence and distribution of DIDS-binding sites of tumorigenic and non-tumorigenic human somatic-cell hybrids. The monoclonal antibody (E8) was produced by the fusion of the mouse myeloma (NS-1) with mouse spleen cells and is of the IgG1 subclass. The apparent half-saturation of DIDS for HEp-2 cells is 16 microM and the reaction is rapid. The number of binding sites on tumorigenic and non-tumorigenic hybrid cells was the same. The DIDS-binding protein occurs homogeneously on all cells, a characteristic which distinguishes it from the possible tumour antigen recognised by the M/27 monoclonal antibody.
摘要
制备了一种针对阴离子转运抑制剂DIDS的单克隆抗体，并用于比较致瘤性和非致瘤性人类体细胞杂种中DIDS结合位点的出现情况和分布。该单克隆抗体（E8）由小鼠骨髓瘤细胞（NS-1）与小鼠脾细胞融合产生，属于IgG1亚类。DIDS对HEp-2细胞的表观半饱和浓度为16微摩尔，反应迅速。致瘤性和非致瘤性杂种细胞上的结合位点数相同。DIDS结合蛋白在所有细胞上均匀存在，这一特征使其与M/27单克隆抗体识别的可能肿瘤抗原区分开来。
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