通过激发能量转移效率波动监测链状分子的分子内动力学。一项理论研究。
Intramolecular dynamics of chain molecules monitored by fluctuations in efficiency of excitation energy transfer. A theoretical study.
作者信息
Haas E, Steinberg I Z
出版信息
Biophys J. 1984 Oct;46(4):429-37. doi: 10.1016/S0006-3495(84)84040-0.
Abstract
The fluorescence quantum yield of a polymer molecule to which an energy donor chromophore and an energy acceptor chromophore are attached depends on the distance between the donor and acceptor chromophores. If this distance fluctuates with time, the fluorescence intensity is expected to fluctuate as well, and the time course of the intensity fluctuations will be correlated with the time course of the changes in the interchromophore distance. The intensity fluctuations are experimentally measurable if the number of illuminated molecules is small. A theoretical treatment of such fluorescence intensity fluctuations is presented in terms of a parameter that describes the polymer chain dynamics. Computer simulations were performed to illustrate the dependence of the autocorrelation function of the intensity fluctuations on the polymer chain conformation, the interchromophore energy transfer properties, and the macromolecular dynamics. These simulations demonstrate that the intensity fluctuations due to nonradiative energy transfer between chromophores attached to polymer chains can be large enough to be experimentally useful in the study of intramolecular dynamics of macromolecules.
摘要
连接有能量供体发色团和能量受体发色团的聚合物分子的荧光量子产率取决于供体和受体发色团之间的距离。如果这个距离随时间波动，那么荧光强度预计也会波动，并且强度波动的时间进程将与发色团间距离变化的时间进程相关。如果被照射分子的数量较少，强度波动在实验上是可测量的。本文根据描述聚合物链动力学的一个参数，对这种荧光强度波动进行了理论处理。进行了计算机模拟，以说明强度波动的自相关函数对聚合物链构象、发色团间能量转移性质和大分子动力学的依赖性。这些模拟表明，连接在聚合物链上的发色团之间的非辐射能量转移引起的强度波动可能足够大，在研究大分子的分子内动力学方面具有实验用途。
相似文献
1
Intramolecular dynamics of chain molecules monitored by fluctuations in efficiency of excitation energy transfer. A theoretical study.通过激发能量转移效率波动监测链状分子的分子内动力学。一项理论研究。
Biophys J. 1984 Oct;46(4):429-37. doi: 10.1016/S0006-3495(84)84040-0.
2
Simultaneous determination of intramolecular distance distributions and conformational dynamics by global analysis of energy transfer measurements.通过能量转移测量的全局分析同时测定分子内距离分布和构象动力学。
Biophys J. 1989 Jun;55(6):1225-36. doi: 10.1016/S0006-3495(89)82918-2.
3
The effect of a distribution of separations upon intramolecular distances in biopolymers, as determined by radiationless energy transfer.
Biophys Chem. 1989 Mar;33(1):71-6. doi: 10.1016/0301-4622(89)80009-2.
4
Distance and orientation dependence of excitation energy transfer: from molecular systems to metal nanoparticles.激发能量转移的距离和方向依赖性：从分子体系到金属纳米颗粒
J Phys Chem B. 2009 Feb 19;113(7):1817-32. doi: 10.1021/jp806536w.
5
Förster-type energy transfer as a probe for changes in local fluctuations of the protein matrix.
Biochemistry. 1984 Jul 17;23(15):3403-11. doi: 10.1021/bi00310a004.
6
Influence of environment induced correlated fluctuations in electronic coupling on coherent excitation energy transfer dynamics in model photosynthetic systems.环境诱导的电子耦合关联涨落对模型光合作用系统中相干激发能量转移动力学的影响。
J Chem Phys. 2012 Mar 21;136(11):115102. doi: 10.1063/1.3693019.
7
Intramolecular electronic excitation energy transfer in donor/acceptor dyads studied by time and frequency resolved single molecule spectroscopy.通过时间分辨和频率分辨单分子光谱研究供体/受体二元体系中的分子内电子激发能转移
J Chem Phys. 2008 Mar 28;128(12):124516. doi: 10.1063/1.2841474.
8
Measuring distances within unfolded biopolymers using fluorescence resonance energy transfer: The effect of polymer chain dynamics on the observed fluorescence resonance energy transfer efficiency.利用荧光共振能量转移测量未折叠生物聚合物内的距离：聚合物链动力学对观察到的荧光共振能量转移效率的影响。
J Chem Phys. 2009 Aug 28;131(8):085105. doi: 10.1063/1.3212602.
9
Statistical interpretation of fluorescence energy transfer measurements in macromolecular systems.大分子系统中荧光能量转移测量的统计学解释
Biochemistry. 1976 May 18;15(10):2105-13. doi: 10.1021/bi00655a012.
10
Temporal switching of homo-FRET pathways in single-chromophore dimer models of π-conjugated polymers.在共轭聚合物的单发色团二聚体模型中，同分子内荧光能量转移途径的时间切换。
J Am Chem Soc. 2013 Jan 9;135(1):78-81. doi: 10.1021/ja3108643. Epub 2012 Dec 26.
引用本文的文献
1
On distributions of barrier crossing times as observed in single-molecule studies of biomolecules.关于生物分子单分子研究中观察到的势垒穿越时间分布。
Biophys Rep (N Y). 2021 Oct 22;1(2):100029. doi: 10.1016/j.bpr.2021.100029. eCollection 2021 Dec 8.
2
The structural heterogeneity of α-synuclein is governed by several distinct subpopulations with interconversion times slower than milliseconds.α-突触核蛋白的结构异质性受几个不同亚群控制，这些亚群之间的转化时间慢于毫秒。
Structure. 2021 Sep 2;29(9):1048-1064.e6. doi: 10.1016/j.str.2021.05.002. Epub 2021 May 19.
3
FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices.基于荧光共振能量转移的动态结构生物学：挑战、前景及对开放科学实践的呼吁
Elife. 2021 Mar 29;10:e60416. doi: 10.7554/eLife.60416.
4
End-to-End Diffusion and Distance Distributions of Flexible Donor-Acceptor Systems Observed by Intramolecular Energy Transfer and Frequency-Domain Fluorometry; Enhanced Resolution by Global Analysis of Externally Quenched and Nonquenched Samples.通过分子内能量转移和频域荧光法观察柔性供体-受体系统的端到端扩散和距离分布；通过对外部猝灭和未猝灭样品的全局分析提高分辨率。
J Phys Chem. 1991 Nov 1;95(24):9654-9660. doi: 10.1021/j100177a012.
5
Toward dynamic structural biology: Two decades of single-molecule Förster resonance energy transfer.迈向动态结构生物学：单分子Förster 共振能量转移的二十年。
Science. 2018 Jan 19;359(6373). doi: 10.1126/science.aan1133.
6
Resolution of Two Sub-Populations of Conformers and Their Individual Dynamics by Time Resolved Ensemble Level FRET Measurements.通过时间分辨整体水平荧光共振能量转移测量解析构象异构体的两个亚群及其个体动力学
PLoS One. 2015 Dec 23;10(12):e0143732. doi: 10.1371/journal.pone.0143732. eCollection 2015.
7
Kinetics of fast changing intramolecular distance distributions obtained by combined analysis of FRET efficiency kinetics and time-resolved FRET equilibrium measurements.通过对 FRET 效率动力学和时间分辨 FRET 平衡测量的综合分析得到的快速变化的分子内距离分布的动力学。
Biophys J. 2014 Feb 4;106(3):667-76. doi: 10.1016/j.bpj.2013.11.4500.
8
Distance distributions and dynamics of a zinc finger peptide from fluorescence resonance energy transfer measurements.荧光共振能量转移测量中锌指肽的距离分布和动力学。
J Fluoresc. 1993 Mar;3(1):23-31. doi: 10.1007/BF00865286.
9
End-to-end diffusion coefficients and distance distributions from fluorescence energy transfer measurements: Enhanced resolution by using multiple donors with different lifetimes.从荧光能量转移测量中获得的端到端扩散系数和距离分布：通过使用具有不同寿命的多个供体提高分辨率。
J Fluoresc. 1995 Jun;5(2):195-203. doi: 10.1007/BF00727540.
10
Protein dynamics from single-molecule fluorescence intensity correlation functions.从单分子荧光强度相关函数看蛋白质动力学。
J Chem Phys. 2009 Sep 7;131(9):095102. doi: 10.1063/1.3212597.
本文引用的文献
1
Evaluation of the distribution of distances between energy donors and acceptors by fluorescence decay.通过荧光衰减评估能量供体与受体之间距离的分布情况。
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2273-7. doi: 10.1073/pnas.69.8.2273.
2
Photon and fluorescence correlation spectroscopy and light scattering of eye-lens proteins at moderate concentrations.中等浓度下眼晶状体蛋白的光子与荧光相关光谱及光散射
Biophys J. 1983 Sep;43(3):345-54. doi: 10.1016/S0006-3495(83)84358-6.
3
The use of fluorescence correlations spectroscopy to probe chromatin in the cell nucleus.利用荧光相关光谱法探测细胞核中的染色质。
Biochim Biophys Acta. 1980 Nov 14;610(1):28-46. doi: 10.1016/0005-2787(80)90053-2.
4
Energy transfer: a spectroscopic ruler.能量转移：一种光谱尺。
Proc Natl Acad Sci U S A. 1967 Aug;58(2):719-26. doi: 10.1073/pnas.58.2.719.
5
Fluorescence correlation spectroscopy. II. An experimental realization.荧光相关光谱学。II. 实验实现。
Biopolymers. 1974 Jan;13(1):29-61. doi: 10.1002/bip.1974.360130103.
6
Fluorescence correlation spectroscopy applied to rotational diffusion of macromolecules.应用于大分子旋转扩散的荧光相关光谱法。
Q Rev Biophys. 1976 Feb;9(1):69-81. doi: 10.1017/s003358350000216x.
7
Applications of fluorescence correlation spectroscopy.
Q Rev Biophys. 1976 Feb;9(1):49-68. doi: 10.1017/s0033583500002158.
8
Concentration correlation spectroscopy: a new biophysical probe based on occupation number fluctuations.
Annu Rev Biophys Bioeng. 1975;4(00):311-34. doi: 10.1146/annurev.bb.04.060175.001523.
9
Distribution of end-to-end distances of oligopeptides in solution as estimated by energy transfer.通过能量转移估计溶液中寡肽的端到端距离分布。
Proc Natl Acad Sci U S A. 1975 May;72(5):1807-11. doi: 10.1073/pnas.72.5.1807.
10
Effect of the orientation of donor and acceptor on the probability of energy transfer involving electronic transitions of mixed polarization.供体和受体的取向对涉及混合极化电子跃迁的能量转移概率的影响。
Biochemistry. 1978 Nov 14;17(23):5064-70. doi: 10.1021/bi00616a032.
Zotero 插件