Allcutt D, Berry M, Sievers J
Brain Res. 1984 Nov;318(2):219-30. doi: 10.1016/0165-3806(84)90027-0.
The response of neurons and glia of the ganglion cell layer of the retina to optic nerve crush was studied in adult and neonatal albino mice between 10-85 days post-lesion (dpl). The numbers of ganglion cells and glia surviving optic nerve transection were quantified using Nissl-stained retinal whole mounts. Large- and intermediate-sized ganglion cells were more sensitive to axotomy in the optic nerve than small-sized cells. About 80% of the former cells degenerated by 10 dpl in adult mice whilst 90-100% were affected in neonates. 40-60% of the small-sized ganglion cells survived in adults whilst in neonates, although a similar number escaped the effects of axotomy at 10 dpl, by 30 dpl only less than 10% remained. These aspects were reflected in the changes in the relative frequency distribution with time of ganglion cells in normal and lesioned mice. No significant alterations in glial cells in the ganglion cell and fibre layers of the retina were recorded at any time of lesioning the optic nerve.
在成年和新生白化小鼠中，研究了视网膜神经节细胞层的神经元和神经胶质细胞在视神经损伤后10 - 85天（损伤后天数，dpl）对视神经挤压的反应。使用尼氏染色的视网膜全层标本对经视神经横断后存活的神经节细胞和神经胶质细胞数量进行定量分析。大型和中型神经节细胞比小型神经节细胞对视神经切断更敏感。在成年小鼠中，约80%的前一类细胞在损伤后10天退化，而在新生小鼠中90 - 100%受影响。在成年小鼠中，40 - 60%的小型神经节细胞存活，而在新生小鼠中，尽管在损伤后10天有相似数量的细胞未受轴突切断影响，但到损伤后30天仅剩下不到10%。这些情况反映在正常和损伤小鼠中神经节细胞相对频率分布随时间的变化上。在视神经损伤的任何时间，视网膜神经节细胞层和纤维层的神经胶质细胞均未记录到显著变化。
