UPMC Eye Center, Eye and Ear Institute, Ophthalmology and Visual Science Research Center, Department of Ophthalmology, University of Pittsburgh School of Medicine, 203 Lothrop Street, Pittsburgh, PA 15213, USA.
Invest Ophthalmol Vis Sci. 2011 Apr 6;52(5):2250-4. doi: 10.1167/iovs.10-6311.
To investigate the longitudinal effect of optic nerve crush injury in mice by measuring retinal thickness with spectral-domain optical coherence tomography (SD-OCT).
Optic nerves of one eye from each C57Bl/6 mouse were crushed under direct visualization for 3 seconds, 1 mm posterior to the globe. The optic nerve head (ONH) was imaged with SD-OCT (1.5 × 1.5 × 2.0 mm scan) before the surgical intervention and repeated subsequently for up to 32 days postinjury. A cohort of mice not exposed to the nerve crush procedure served as control. En face SD-OCT images were used to manually align subsequent scans to the baseline en face image. Total retinal thickness (TRT) (along a sampling band with radii 0.33-0.42 mm centered on the ONH) from each follow-up day was automatically quantified for global and sectoral measurements using custom software. Linear mixed-effects models with quadratic terms were fitted to compare TRT of nerve-crushed and control eyes over time.
Eleven eyes from 11 nerve crush mice (baseline age 76 ± 11.8 days) and eight eyes from four healthy mice (baseline age 64 ± 0 days) were included. The control eyes showed a small, gradual, and consistent TRT increase throughout follow-up. Nerve-crushed eyes showed an initial period of thickening, followed by thinning and slight rebound after day 21. The decrease in thickness observed after the early thickening resolved was statistically significantly different from the control eyes (P < 0.05 for global and sectoral measurements).
SD-OCT can be used to quantitatively monitor changes in retinal thickness in mice over time.
通过频域光相干断层扫描（SD-OCT）测量视网膜厚度，研究小鼠视神经挤压伤的纵向效应。
在直视下，用 SD-OCT（1.5×1.5×2.0mm 扫描）对每只 C57Bl/6 小鼠一只眼的视神经进行 3 秒、球后 1mm 的挤压。在手术干预前和损伤后最多 32 天内对视神经盘（ONH）进行 SD-OCT 成像。一组未暴露于神经挤压程序的小鼠作为对照。使用 En face SD-OCT 图像手动将后续扫描与基线 En face 图像对齐。使用定制软件自动对每个后续测量日的总视网膜厚度（TRT）（沿以 ONH 为中心的半径为 0.33-0.42mm 的采样带）进行全局和扇形测量。使用带有二次项的线性混合效应模型来比较神经挤压眼和对照眼随时间的 TRT。
11 只视神经挤压鼠（基线年龄 76±11.8 天）的 11 只眼和 4 只健康鼠（基线年龄 64±0 天）的 8 只眼被纳入研究。对照眼在整个随访过程中表现出微小、逐渐且一致的 TRT 增加。视神经挤压眼表现为早期增厚，随后在第 21 天后变薄并略有反弹。早期增厚后观察到的厚度减少与对照眼明显不同（全局和扇形测量的 P 值均<0.05）。
SD-OCT 可用于定量监测小鼠视网膜厚度随时间的变化。
