Robins H I, Dennis W H, Martin P A, Sondel P M, Yatvin M B, Steeves R A
Cancer. 1984 Dec 15;54(12):2831-5. doi: 10.1002/1097-0142(19841215)54:12<2831::aid-cncr2820541202>3.0.co;2-8.
Previous work has utilized spleen colony formation to evaluate the fractional survival of AKR leukemia and normal bone marrow cells after in vitro heat exposure. An inherently greater sensitivity of neoplastic cells to thermal killing, as compared to normal syngeneic stem cells, has been established both at 41.8 degrees C and 42.5 degrees C. Normal bone marrow colony-forming units were assayed in lethally irradiated (750 cGy) mice. Leukemic colony-forming units were assayed in nonirradiated mice. Using this methodology, the authors demonstrated that the differential effect of hyperthermia on AKR murine leukemia and AKR bone marrow cells can be further enhanced by the addition of lidocaine or thiopental to incubation mixtures. These findings may have application to autologous bone marrow transplantation in humans.
先前的研究利用脾集落形成来评估体外热暴露后AKR白血病细胞和正常骨髓细胞的存活分数。与正常同基因干细胞相比，在41.8摄氏度和42.5摄氏度时，肿瘤细胞对热杀伤具有固有的更高敏感性。在接受致死剂量照射（750 cGy）的小鼠中检测正常骨髓集落形成单位。在未照射的小鼠中检测白血病集落形成单位。使用这种方法，作者证明，通过在孵育混合物中添加利多卡因或硫喷妥钠，热疗对AKR小鼠白血病和AKR骨髓细胞的差异效应可以进一步增强。这些发现可能适用于人类自体骨髓移植。
