Robins H I, Steeves R A, Clark A W, Martin P A, Miller K, Dennis W H
Cancer Res. 1983 Oct;43(10):4951-5.
To determine if there is a differential effect of hyperthermia on AKR murine leukemia and AKR normal bone marrow cells incubated in vitro, the fractional survival of leukemic and of normal cells with proliferative potential as a function of heating exposure was estimated by evaluating spleen colony formation. Normal bone marrow colony-forming units were assayed in lethally irradiated (750 centigrays) mice; leukemic colony-forming units were assayed in nonirradiated mice. Electron micrographic studies of leukemic cells treated with 41.8 degrees hyperthermia found that structural damage to the cell, i.e., changes in the Golgi apparatus, was associated with the lack of ability to form colonies. AKR leukemia cells were more sensitive than normal cells to hyperthermic killing at 41.8 degrees and at 42.5 degrees. This differential was found whether cells of each type were heated separately or when mixed together. This model system demonstrates an inherently greater sensitivity of neoplastic cells, as compared to normal syngeneic stem cells, to thermal killing. This finding may have relevance to autologous bone marrow transplantation in humans.
为了确定热疗对体外培养的AKR小鼠白血病细胞和AKR正常骨髓细胞是否有不同影响，通过评估脾集落形成来估计具有增殖潜力的白血病细胞和正常细胞的存活分数与热暴露的关系。正常骨髓集落形成单位在接受致死剂量照射（750厘戈瑞）的小鼠中进行测定；白血病集落形成单位在未照射的小鼠中进行测定。对经41.8℃热疗处理的白血病细胞进行电子显微镜研究发现，细胞的结构损伤，即高尔基体的变化，与形成集落的能力缺乏有关。AKR白血病细胞在41.8℃和42.5℃时比正常细胞对热杀伤更敏感。无论每种类型的细胞是单独加热还是混合在一起加热，都发现了这种差异。该模型系统表明，与正常同基因干细胞相比，肿瘤细胞对热杀伤具有固有的更高敏感性。这一发现可能与人类自体骨髓移植有关。
