Robins H I, Steeves R A, Schmitt C L, Peterson C, Martin P A
University of Wisconsin Clinical Cancer Center, Madison 53792.
Int J Radiat Oncol Biol Phys. 1988 May;14(5):979-82. doi: 10.1016/0360-3016(88)90021-1.
It has been previously demonstrated that AKR leukemia (lymphoma) cells are more sensitive than normal bone marrow cells to hyperthermic killing at 41.8 degrees C and 42.5 degrees C in vitro. This differential heat sensitivity might be explained by a greater ability to induce thermotolerance (TT) in normal versus neoplastic hematopoetic cells. We tested this hypothesis using the spleen colony methodology in the AKR murine model. A greater heat sensitivity of leukemia in comparison to normal bone marrow cells was observed at 42.5 degrees C; this observation agrees with previous reports. However, using a preincubation temperature of 40.0 degrees C for 120 min did not result in the induction of TT in either normal bone marrow (AKR) cells or AKR leukemia cells. The rationale for the choice of preincubation temperatures and times, as well as the clinical implications of these results are discussed.
先前已证明，在体外41.8摄氏度和42.5摄氏度条件下，AKR白血病（淋巴瘤）细胞比正常骨髓细胞对热杀伤更敏感。这种不同的热敏感性可能是由于正常造血细胞与肿瘤造血细胞相比具有更强的诱导热耐受（TT）的能力。我们在AKR小鼠模型中使用脾集落法对这一假设进行了测试。在42.5摄氏度时，观察到白血病细胞比正常骨髓细胞对热更敏感；这一观察结果与先前的报道一致。然而，在40.0摄氏度预孵育120分钟，无论是正常骨髓（AKR）细胞还是AKR白血病细胞都未诱导出热耐受。文中讨论了选择预孵育温度和时间的基本原理以及这些结果的临床意义。
