Ishii K, Sakamoto H, Furuyama J, Hanaoka M
Cell Struct Funct. 1984 Jun;9(2):117-23. doi: 10.1247/csf.9.117.
We developed a test medium for cytidine deaminase in order to examine the distribution of this enzyme in cultured cell lines. The growth of various mammalian cell lines was tested in culture medium containing 2 microM pyrazofurin and 100 microM cytidine. Enzymological assays for the enzyme also were made spectrophotometrically with cell extracts. A good correlation was found between results of cell growth tests and the levels of enzyme activity. Twelve of twenty cell lines were killed in the test medium, but the remaining lines showed good growth. The levels of enzyme activities were lower in the former lines than in the latter. The critical level of enzyme activity required to support cell growth was approximately 30 units per mg protein. These findings indicate that culture medium containing 2 microM pyrazofurin and 100 microM cytidine serves as a test medium for cytidine deaminase. The possibility that the cytidine deaminase may be useful in determining the embryonic origin of cultured cell lines is discussed, based on the growth properties of various cultured cell lines in the test medium.
我们开发了一种用于胞苷脱氨酶的测试培养基，以检测该酶在培养细胞系中的分布。在含有2微摩尔吡唑呋林和100微摩尔胞苷的培养基中测试了各种哺乳动物细胞系的生长情况。还用细胞提取物通过分光光度法对该酶进行了酶学测定。细胞生长测试结果与酶活性水平之间发现了良好的相关性。20个细胞系中有12个在测试培养基中死亡，但其余细胞系生长良好。前一组细胞系中的酶活性水平低于后一组。支持细胞生长所需的酶活性临界水平约为每毫克蛋白质30单位。这些发现表明，含有2微摩尔吡唑呋林和100微摩尔胞苷的培养基可作为胞苷脱氨酶的测试培养基。基于各种培养细胞系在测试培养基中的生长特性，讨论了胞苷脱氨酶在确定培养细胞系的胚胎起源方面可能有用的可能性。
