Fischer T, Maibach H
Contact Dermatitis. 1984 Sep;11(3):137-40. doi: 10.1111/j.1600-0536.1984.tb00957.x.
Proper technique aids reliable patch test results. The Finn chamber volume was determined to be 25 microliters. These studies suggest the optimal volume semi-solid test substance to be 12-15 microliters. More than 18-21 microliters caused significant extrusion of test substance and improper adhesive adherence. Finn chamber paper discs used with solutions absorb a maximum of 17.5 microliters test solutions and keep 15 microliters within the chamber. The evaporation rate of aqueous test solutions from Finn chambers was 1 mg/3 min; thus, the chambers should be applied quickly to avoid drying.
正确的技术有助于获得可靠的斑贴试验结果。芬恩小室的容积经测定为25微升。这些研究表明，半固体试验物质的最佳体积为12 - 15微升。超过18 - 21微升会导致试验物质显著挤出以及粘贴不适当。与溶液一起使用的芬恩小室纸盘最多可吸收17.5微升试验溶液，并在小室内保留15微升。来自芬恩小室的水性试验溶液的蒸发速率为1毫克/3分钟；因此，应迅速粘贴小室以避免干燥。
