淋巴细胞分离的各种方法及其与非免疫性玫瑰花结形成的相关性。

Various methods of lymphocyte separation and their relevance with the formation of non-immune rosettes.

作者信息

Phatak A G

出版信息

J Immunol Methods. 1978;20:109-15. doi: 10.1016/0022-1759(78)90249-1.

Abstract

Lymphocytes (leukocytes mainly containing mononuclear cells) are separated by six different methods and these lymphocytes are used for non-immune rosette formation. It is found that the percentage of non-immune rosettes varies according to the method of separation of lymphocytes. Buffy coat on Dextran-70 and Gravity Sedimentation method give the best rosette yields as compared with lymphocytes separated by centrifugation of heparinized blood (unsupported centrifugation) P less than 0.001) or on Hypaque-Ficoll or Diaginol-Ficoll gradients (supported centrifugation) P less than 0.05). Furthermore, the buffy coat separated on Dextran-70, layered on Ficoll-gradients (supported centrifugation) yields lower values of rosettes (P less than 0.05). It is possible that in certain methods which yield lower values of rosettes there is loss of selected subpopulations of lymphocytes. This loss of subpopulation appears to be due to the centrifugation of the blood and the loss is appreciably more on 'unsupported' than 'supported' centrifugation.

摘要

淋巴细胞（主要含单核细胞的白细胞）通过六种不同方法进行分离，这些淋巴细胞用于非免疫性玫瑰花结的形成。研究发现，非免疫性玫瑰花结的百分比会因淋巴细胞的分离方法而异。与通过肝素化血液离心分离的淋巴细胞（无支持离心，P<0.001）或在聚蔗糖-泛影葡胺或泛影葡胺-聚蔗糖梯度上分离的淋巴细胞（有支持离心，P<0.05）相比，葡聚糖-70上的血沉棕黄层和重力沉降法产生的玫瑰花结产量最高。此外，在葡聚糖-70上分离的血沉棕黄层，铺在聚蔗糖梯度上（有支持离心）产生的玫瑰花结值较低（P<0.05）。在某些产生较低玫瑰花结值的方法中，可能存在特定淋巴细胞亚群的丢失。这种亚群的丢失似乎是由于血液的离心，并且“无支持”离心比“有支持”离心的丢失明显更多。