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[静止生长期HeLa肿瘤细胞在低强度红光照射后传代培养期间的生长动力学]

[Growth kinetics of HeLa tumor cells during subculturing after irradiation with low-intensity red light in the stationary growth phase].

作者信息

Karu T I, Kalendo G S, Lobko V V, Piatibrat L V

出版信息

Eksp Onkol. 1984;6(4):60-2.

PMID:6499755
Abstract

The curves of relative growth rate of HeLa cells were studied after their irradiation at the stationary growth phase (He-Ne laser, lambda = 632.8 nm, D = 100 J/m2) and reinoculation to the fresh medium in different periods after the irradiation. The irradiation evokes changes in cells of the stationary phase which are realized in subcultivation. When the interval between irradiation and inoculation is short (5 min), the cell growth is inhibited; when the interval becomes longer (up to 4 h), the cell growth in the exponential phase increases. In the HeLa cell culture with the stimulated growth only a part of cells are able, to reach the stationary growth phase.

摘要

研究了处于稳定生长期的HeLa细胞在接受照射(氦氖激光,波长λ = 632.8 nm,剂量D = 100 J/m²)后,于照射后不同时间段重新接种到新鲜培养基中的相对生长速率曲线。照射会引起稳定期细胞的变化,这些变化在传代培养中得以体现。当照射与接种之间的间隔较短(5分钟)时,细胞生长受到抑制;当间隔变长(长达4小时)时,指数生长期的细胞生长增加。在生长受到刺激的HeLa细胞培养物中,只有一部分细胞能够达到稳定生长期。

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