Beagley K W, Horne L S, Noronha R F, Goodall C M, Moore C M
In Vitro. 1984 Aug;20(8):623-8. doi: 10.1007/BF02619611.
In vitro growth of rat atriocaval epithelial tumor cells (ACT-1) was enhanced by the inclusion of xenogeneic mouse adherent peritoneal exudate cells (PECs) in a two-layer soft agar system. A linear relationship was found between the number of cells plated and the number of colonies when ACT-1 tumor cells were plated at plating densities of between 1 and 5 X 10(5) cell/60 mm plate (r = 0.9, P less than 0.001). Inclusion of irradiated PECs in the bioassay for tumor stem cells resulted in a two and a half-fold increase in colony formation in three separate experiments (P less than 0.001).
在两层软琼脂系统中加入异种小鼠贴壁腹膜渗出细胞（PEC）可增强大鼠心房静脉上皮肿瘤细胞（ACT-1）的体外生长。当ACT-1肿瘤细胞以1至5×10⁵个细胞/60毫米培养皿的接种密度接种时，接种的细胞数量与集落数量之间呈线性关系（r = 0.9，P<0.001）。在肿瘤干细胞生物测定中加入经辐照的PEC，在三个独立实验中集落形成增加了2.5倍（P<0.001）。
