Hamburger A W, White C P, Dunn F E
Br J Cancer. 1983 Nov;48(5):675-82. doi: 10.1038/bjc.1983.249.
The ability of human tumour cells to form colonies in soft agar is enhanced by the presence of autologous phagocytic/adherent cells. We investigated the effect of irradiation on the ability of the adherent cells to support human tumour colony formation. Relatively low doses of irradiation significantly increased the growth enhancing ability of adherent cells in 17/19 cases. The possibility that the enhancement was mediated by inactivation of radiosensitive contaminating lymphocytes was explored. Depletion of T lymphocytes from unirradiated adherent cells by a monoclonal antibody and complement resulted in little overall change in tumour colony growth. However, elimination of only the suppressor subset (OKT8+) of T lymphocytes resulted in increased colony growth relative to control values obtained with unirradiated adherent cells. In contrast, depletion of T lymphocytes from irradiated adherent cells by a pan T monoclonal antibody and complement decreased colony formation. Thus, the ability of irradiated macrophages to enhance tumour colony growth appeared to be mediated by a T lymphocyte. The effect of irradiation on isolated populations of macrophages and T lymphocytes was also examined. The enhanced ability of irradiated adherent cells to support tumor colony growth appeared to have been due to treatment of T lymphocytes alone. The results indicate that both adherent macrophages and lymphocytes may influence the growth of clonogenic human tumour cells.
自体吞噬/黏附细胞的存在可增强人肿瘤细胞在软琼脂中形成集落的能力。我们研究了辐射对黏附细胞支持人肿瘤集落形成能力的影响。相对低剂量的辐射在19例中有17例显著提高了黏附细胞的生长增强能力。我们探讨了这种增强作用是否由放射敏感的污染淋巴细胞失活介导。用单克隆抗体和补体去除未照射的黏附细胞中的T淋巴细胞，肿瘤集落生长总体变化不大。然而，仅去除T淋巴细胞的抑制性子集（OKT8+）导致集落生长相对于未照射黏附细胞获得的对照值增加。相反，用泛T单克隆抗体和补体去除照射后黏附细胞中的T淋巴细胞会减少集落形成。因此，照射后巨噬细胞增强肿瘤集落生长的能力似乎由T淋巴细胞介导。我们还研究了辐射对分离的巨噬细胞和T淋巴细胞群体的影响。照射后黏附细胞支持肿瘤集落生长的能力增强似乎仅归因于对T淋巴细胞的处理。结果表明，黏附的巨噬细胞和淋巴细胞都可能影响人肿瘤克隆细胞的生长。
