Stewart C C, Beetham K L
Int J Cancer. 1978 Aug 15;22(2):152-9. doi: 10.1002/ijc.2910220208.
The EMT6 mouse tumor was selected for use in the study of cytocidal activity and proliferative ability of infiltrating macrophages because of its high plating efficiency when explanted to culture. The plating efficiency for cells directly plated in culture from the tumor was 28 +/- 9.3%, irrespective of the size of the tumor. Of the adherent cells derived from the tumor, the fraction that was macrophages increased from 27% at 7 days to 47% at 28 days after initial injection. Time-lapse cinemicrography was used to directly observe adherent cells derived from the tumor, and macrophages were found to be cytocidal. When grown in the presence of L-cell conditioned medium no macrophage colonies were found when cultures were established from untreated mice even though most tumor cell colonies contained macrophages. When mice were first treated with 0.8 mg BCNU prior to establishment of the cultures, in order to reduce the frequency of colony-forming tumor cells, approximately half the colonies found contained only macrophages. These results show that macrophages in this tumor are cytocidal and capable of proliferation.
选择EMT6小鼠肿瘤用于研究浸润巨噬细胞的杀伤活性和增殖能力，因为将其接种到培养基中时具有较高的接种效率。直接从肿瘤接种到培养基中的细胞接种效率为28±9.3%，与肿瘤大小无关。在最初注射后，从肿瘤衍生的贴壁细胞中，巨噬细胞的比例从第7天的27%增加到第28天的47%。采用延时电影显微镜直接观察肿瘤衍生的贴壁细胞，发现巨噬细胞具有杀伤作用。当在L细胞条件培养基存在下培养时，从未经处理的小鼠建立培养物时未发现巨噬细胞集落，尽管大多数肿瘤细胞集落中含有巨噬细胞。为了降低形成集落的肿瘤细胞频率，在建立培养物之前先用0.8mg卡莫司汀处理小鼠，发现大约一半的集落仅含有巨噬细胞。这些结果表明，该肿瘤中的巨噬细胞具有杀伤作用且能够增殖。
