Kundu S K, Roy S K
J Lipid Res. 1978 Mar;19(3):390-5.
DEAE-silica gel has been shown to be an improvement over DEAE-Sephadex for the quantitative isolation of gangliosides and neutral glycosphingolipids from animal tissues or cells. Preliminary results indicated that it can also be used for protein separation. Direct comparative studies of DEAE-silica gel with DEAE-Sephadex showed preferences for the former for the following reasons: i) faster flow rate; ii) more rapid equilibration with the starting buffer; iii) easier regeneration; iv) more economical; and v) a lesser susceptibility to microbial attack.
已证明，对于从动物组织或细胞中定量分离神经节苷脂和中性糖鞘脂，二乙氨基乙基硅胶（DEAE-硅胶）比二乙氨基乙基葡聚糖凝胶（DEAE-葡聚糖凝胶）更具优势。初步结果表明，它也可用于蛋白质分离。对DEAE-硅胶和DEAE-葡聚糖凝胶的直接比较研究表明，由于以下原因，前者更受青睐：i）流速更快；ii）与起始缓冲液的平衡更快；iii）再生更容易；iv）更经济；v）对微生物侵袭的敏感性更低。
