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本文引用的文献

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Degradation of 4-chlorophenylacetic acid by a Pseudomonas species.一株假单胞菌对4-氯苯乙酸的降解作用
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[Microbial degradation and 4-chlorophenylacetic acid. Chemical synthesis of 3-chloro-4-hydroxy-, 4-chloro-3-hydroxy- and 4-chloro-2-hydroxyphenylacetic acid (author's transl)].[微生物降解与4-氯苯乙酸。3-氯-4-羟基、4-氯-3-羟基和4-氯-2-羟基苯乙酸的化学合成（作者译）]
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Oxidation of naphthalene by a multicomponent enzyme system from Pseudomonas sp. strain NCIB 9816.假单胞菌属菌株NCIB 9816的多组分酶系统对萘的氧化作用
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2-Haloacid dehalogenase from a 4-chlorobenzoate-degrading Pseudomonas spec. CBS 3.来自一株降解4-氯苯甲酸的假单胞菌属CBS 3的2-卤酸脱卤酶。
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The prophylactic treatment tuberculosis.预防性治疗肺结核。 （注：原英文表述不太准确规范，推测可能是“The prophylactic treatment of tuberculosis.” ）
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[Degradation of 4-chlorobenzoic acid by an Arthrobacter species (author's transl)].节杆菌属细菌对4-氯苯甲酸的降解作用（作者译）
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Toluene dioxygenase: a multicomponent enzyme system.甲苯双加氧酶：一种多组分酶系统。
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8
Purification and some properties of a soluble benzene-oxidizing system from a strain of Pseudomonas.一株假单胞菌中可溶性苯氧化系统的纯化及某些性质
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来自假单胞菌属菌株CBS3的双组分酶系统对4-氯苯乙酸酯的氧化和脱卤作用

Oxidation and dehalogenation of 4-chlorophenylacetate by a two-component enzyme system from Pseudomonas sp. strain CBS3.

作者信息

Markus A, Klages U, Krauss S, Lingens F

出版信息

J Bacteriol. 1984 Nov;160(2):618-21. doi: 10.1128/jb.160.2.618-621.1984.

DOI:10.1128/jb.160.2.618-621.1984
PMID:6501216
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC214779/
Abstract

In cell-free extracts from Pseudomonas sp. strain CBS3 the conversion of 4-chlorophenylacetate to 3,4-dihydroxyphenylacetate was demonstrated. By Sephacryl S-200 chromatography two protein fractions, A and B, were obtained which both were essential for enzyme activity. Fe2+ and NADH were cofactors of the reaction. NADPH also activated the enzyme, but less effectively than NADH. FAD had no influence on enzyme activity. 4-Hydroxyphenylacetate, 4-chloro-3-hydroxyphenylacetate, and 3-chloro-4-hydroxyphenylacetate were poor substrates for the enzyme, suggesting that these substances are not intermediates of the reaction. We therefore suggest that the reaction proceeds via a dioxygenated intermediate.

摘要

在来自假单胞菌属菌株CBS3的无细胞提取物中，证明了4-氯苯乙酸向3,4-二羟基苯乙酸的转化。通过Sephacryl S-200色谱法获得了两个蛋白质组分A和B，它们对于酶活性都是必需的。Fe2+和NADH是该反应的辅因子。NADPH也能激活该酶，但效果不如NADH。FAD对酶活性没有影响。4-羟基苯乙酸、4-氯-3-羟基苯乙酸和3-氯-4-羟基苯乙酸是该酶的较差底物，这表明这些物质不是该反应的中间体。因此，我们认为该反应通过双加氧中间体进行。