Vanderslice J T, Higgs D J
J Chromatogr Sci. 1984 Nov;22(11):485-9. doi: 10.1093/chromsci/22.11.485.
A high performance liquid chromatographic (HPLC) procedure has been developed for the analysis of ascorbic acid and dehydroascorbic acid in complex matrices. Separation is accomplished with an anion-exchange resin and fluorescent detection is achieved through post-column inline chemistry, involving oxidation of ascorbic acid to dehydroascorbic acid followed by reaction with o-phenylenediamine to form a fluorescent product. Lower limits of detection for both forms of vitamin C are well below the levels found in the usual food sources of this vitamin. The extraction procedures developed yield clean samples for analysis with minimal loss of the vitamers during the analytical procedures. Recoveries are in the range of 90-107%. The results obtained with this HPLC procedure agree well with those obtained with a modified version of the classical procedure of Deutsch and Weeks. A variety of foods including fruit juices, vegetables, and fruits were analyzed.
已开发出一种高效液相色谱（HPLC）方法，用于分析复杂基质中的抗坏血酸和脱氢抗坏血酸。使用阴离子交换树脂进行分离，并通过柱后在线化学方法实现荧光检测，该方法包括将抗坏血酸氧化为脱氢抗坏血酸，然后与邻苯二胺反应形成荧光产物。两种形式的维生素C的检测下限远低于该维生素常见食物来源中的含量。所开发的提取程序可得到用于分析的纯净样品，在分析过程中维生素异构体的损失最小。回收率在90 - 107%范围内。该HPLC方法得到的结果与Deutsch和Weeks经典方法的改进版本所得到的结果非常吻合。分析了包括果汁、蔬菜和水果在内的多种食物。
