Bilic N
Federal Dairy Research Institute, Liebefeld-Berne, Switzerland.
J Chromatogr. 1991 May 10;543(2):367-74. doi: 10.1016/s0021-9673(01)95788-7.
A procedure for the simultaneous determination of both ascorbic and dehydroascorbic acid in dairy foods by high-performance liquid chromatography using precolumn derivatization with 4-methoxy- and 4-ethoxy-1,2-phenylenediamine is presented. The derivatives are isolated by solid-phase extraction and analysed by fluorescence detection on a resin-type reversed-phase column at pH 9. Retention times are 2 and 3.2 min for the derivatives of ascorbic and dehydroascorbic acid, respectively. Relative standard deviations of the within- and between-assay tests are 7.1 and 5.5%, respectively, for ascorbic and 11 and 9%, respectively, dehydroascorbic acid. The limits of detection are 50 and 70 fmol per 5-microl injection for ascorbic and dehydroascorbic acid, respectively.
本文介绍了一种采用高效液相色谱法，使用4-甲氧基-和4-乙氧基-1,2-苯二胺进行柱前衍生化，同时测定乳制品中抗坏血酸和脱氢抗坏血酸的方法。衍生物通过固相萃取分离，并在pH 9的树脂型反相柱上通过荧光检测进行分析。抗坏血酸和脱氢抗坏血酸衍生物的保留时间分别为2分钟和3.2分钟。抗坏血酸和脱氢抗坏血酸的批内和批间测试相对标准偏差分别为7.1%和5.5%以及11%和9%。抗坏血酸和脱氢抗坏血酸的检测限分别为每5微升进样50和70飞摩尔。
