Nuttall P A, Moss S R, Jones L D, Carey D
J Virol Methods. 1984 Aug;9(1):27-33. doi: 10.1016/0166-0934(84)90080-6.
Three methods of isolating viruses from 10 tick pools were compared; none of the methods produced all 13 of the viruses isolated (7 viruses of the bunyaviridae and 6 orbiviruses). Inoculation of homogenised ticks into various cell lines was the most successful, yielding 11 virus isolations. Only 2 tick homogenates induced overt signs of infection following intra-cerebral inoculation of 2-day-old mice. However, when inoculated mouse brain was passaged in various cell lines, 8 of 12 isolations were made. The rates of success of the 3 methods of virus isolation appeared to vary according to the type and titre of the virus in the tick pool.
对从10组蜱虫样本中分离病毒的三种方法进行了比较；没有一种方法能分离出所有13种病毒（7种布尼亚病毒科病毒和6种环状病毒）。将蜱虫匀浆接种到各种细胞系中是最成功的方法，分离出了11种病毒。只有2份蜱虫匀浆在接种2日龄小鼠脑内后引起明显的感染迹象。然而，当对接种的小鼠脑在各种细胞系中传代时，12次分离中有8次成功。病毒分离的这三种方法的成功率似乎因蜱虫样本中病毒的类型和滴度而异。
