Sonnino S, Ghidoni R, Fiorilli A, Venerando B, Tettamanti G
J Neurosci Res. 1984;12(2-3):193-204. doi: 10.1002/jnr.490120207.
The sialic acid moiety of rat brain cytosolic gangliosides was radiolabeled by intracranial injection of N-(3H)acetylmannosamine. Upon ammonium sulphate fractionation, Sepharose 6B gel filtration, and hydroxylapatite-cellulose chromatography, ganglioside-bound radioactivity of brain cytosolic extract followed the behavior of protein and not that of purified gangliosides. This indicates that cytosolic gangliosides occur as ganglioside-protein complexes. By application of hydroxylapatite-cellulose column chromatography, fractions were obtained having different ganglioside composition. In particular, one fraction contained GM1, one GD1a, and one GT1b with a ganglioside homogeneity better than 95% in each fraction. This indicates the occurrence in brain cytosol of a GM1-protein complex, a GD1a-protein complex, and a GT1b-protein complex.
通过颅内注射N-（3H）-乙酰甘露糖胺对大鼠脑细胞溶胶神经节苷脂的唾液酸部分进行放射性标记。经硫酸铵分级分离、琼脂糖6B凝胶过滤和羟基磷灰石-纤维素色谱分析后，脑细胞溶胶提取物中与神经节苷脂结合的放射性活性遵循蛋白质的行为，而非纯化神经节苷脂的行为。这表明细胞溶胶神经节苷脂以神经节苷脂-蛋白质复合物的形式存在。通过应用羟基磷灰石-纤维素柱色谱法，获得了具有不同神经节苷脂组成的级分。特别是，一个级分含有GM1、一个GD1a和一个GT1b，每个级分中神经节苷脂的纯度均高于95%。这表明在脑细胞溶胶中存在GM1-蛋白质复合物、GD1a-蛋白质复合物和GT1b-蛋白质复合物。
