Ando S, Yu R K
J Neurosci Res. 1984;12(2-3):205-11. doi: 10.1002/jnr.490120208.
A series of major and minor ganglioside species were isolated from a single human adult brain and analyzed for their lipophilic composition. Hematosides, GM3 and GD3, each showing double bands on thin-layer chromatograms, were separated into the upper band and lower band fractions to be analyzed for the heterogeneity of their ceramide moieties. The upper band fractions of GM3 and GD3 were found to contain relatively high amounts of longer-chain fatty acids (C20-C26), whereas the lower band fractions contained high amounts of shorter-chain fatty acids (C16-C18). Compared to other gangliosides which contain hexosamine, the two hematosides contained a smaller proportion of stearic acid. The proportion of 4-eicosasphingenine was found to increase with increasing sialic acid content in gangliosides. These findings suggest that, in the biosynthesis of various gangliosides, N-acetylhexosamine is preferentially transferred to hematosides rich in stearic acid, and sialic acid residues are preferentially transferred to 4-eicosasphingenine-containing species.
从一个成年人的单一脑样本中分离出一系列主要和次要的神经节苷脂种类，并对其亲脂性成分进行分析。在薄层色谱图上均显示出两条带的血型神经节苷脂、GM3和GD3，被分离为上带和下带部分，以分析其神经酰胺部分的异质性。发现GM3和GD3的上带部分含有相对大量的长链脂肪酸（C20 - C26），而下带部分含有大量的短链脂肪酸（C16 - C18）。与其他含有己糖胺的神经节苷脂相比，这两种血型神经节苷脂含有的硬脂酸比例较小。发现4 - 二十碳鞘氨醇的比例随着神经节苷脂中唾液酸含量的增加而增加。这些发现表明，在各种神经节苷脂的生物合成中，N - 乙酰己糖胺优先转移到富含硬脂酸的血型神经节苷脂中，而唾液酸残基优先转移到含4 - 二十碳鞘氨醇的种类中。
