Thiermann A B
Mod Vet Pract. 1984 Oct;65(10):758-9.
Though the microscopic agglutination test is commonly used as an aid to diagnosis of leptospirosis, it lacks sensitivity, cannot differentiate vaccinal titers from those of infection, and cannot identify shedders. The ELISA is sensitive but also cannot differentiate titers of vaccination and infection. Leptospire isolation is essential for a final diagnosis. Tissue or fluid samples should be aseptically collected, if possible, and quickly shipped to the diagnostic laboratory in transport medium or on ice, but should not be frozen. Samples to be collected may include urine, milk, kidney, aqueous humor, and fallopian tube or uterine mucosa.
尽管显微镜凝集试验通常用于辅助诊断钩端螺旋体病，但它缺乏敏感性，无法区分疫苗接种产生的抗体滴度和感染产生的抗体滴度，也无法识别排菌动物。酶联免疫吸附测定法很敏感，但同样无法区分疫苗接种和感染的抗体滴度。钩端螺旋体的分离对于最终诊断至关重要。如果可能的话，应无菌采集组织或液体样本，并迅速用运输培养基或在冰上运送到诊断实验室，但不应冷冻。要采集的样本可能包括尿液、乳汁、肾脏、房水以及输卵管或子宫黏膜。
