Nechipurenko Iu D
Mol Biol (Mosk). 1984 Jul-Aug;18(4):1066-80.
Cooperative effects arising upon binding of biologically active ligands to DNA are considered. Equations are derived which enable one to describe the binding of two different ligands to DNA. We also consider the case when ligand can form two type of DNA complexes. The cooperative binding of the ligand in the vicinity of saturation level of binding can be described with a good accuracy by equation derived for the non-cooperative adsorption of the same ligand with some effective binding constant Keff. It is shown that cooperative effects arising upon binding of proteins and other ligands to DNA can be divided into two groups depending on the symmetry of interactions between the bound ligand molecules. In particular, if such interactions favor the formation of dimeric ligand species on the DNA, Keff approximately a1/2, where a is the ligand-ligand interaction constant. If cooperative interactions favor the formation of aggregates of unrestricted size, then Keff approximately aL+Y, where L is the size of the binding site for the ligand on DNA.
考虑了生物活性配体与DNA结合时产生的协同效应。推导了能够描述两种不同配体与DNA结合的方程。我们还考虑了配体可形成两种类型DNA复合物的情况。在结合饱和水平附近配体的协同结合可以通过为相同配体的非协同吸附推导的方程，用某个有效结合常数Keff相当准确地进行描述。结果表明，蛋白质和其他配体与DNA结合时产生的协同效应可根据结合配体分子间相互作用的对称性分为两组。特别地，如果这种相互作用有利于在DNA上形成二聚体配体物种，则Keff约为a1/2，其中a是配体-配体相互作用常数。如果协同相互作用有利于形成无限制大小的聚集体，则Keff约为aL+Y，其中L是DNA上配体结合位点的大小。
