Nechipurenko Iu D, Zasedatelev A S, Gurskiĭ G V
Mol Biol (Mosk). 1984 May-Jun;18(3):798-812.
Equations are derived to describe the cooperative binding of large ligands to DNA. A mathematical approach is developed which enables one to give a simple probabilistic interpretation of binding equations and to solve them in the general case when long-range interactions are allowed between bound ligands. These interactions can be mediated by conformation changes induced in the DNA in the course of binding process and transformed over some distances beyond the DNA region immediately covered by a bound ligand molecule (allosteric effect of DNA). Interactions between ligand molecules can be formally described in terms of model potential characterizing pairwise interactions between bound ligands. A procedure is developed which allows one to determined the form of such potential from experimentally measured binding isotherms. It is based on a comparison of experimental binding isotherms with the appropriate curves calculated for the case of non-interacting ligands.
推导了用于描述大配体与DNA协同结合的方程。开发了一种数学方法，使人们能够对结合方程给出简单的概率解释，并在允许结合配体之间存在长程相互作用的一般情况下求解这些方程。这些相互作用可以由结合过程中DNA诱导的构象变化介导，并在超出结合配体分子直接覆盖的DNA区域的一定距离上传递（DNA的变构效应）。配体分子之间的相互作用可以用表征结合配体之间成对相互作用的模型势来形式化描述。开发了一种程序，可根据实验测量的结合等温线确定这种势的形式。它基于将实验结合等温线与非相互作用配体情况下计算的相应曲线进行比较。
