Delage G, Nahon E, Huynh T, Jeusset J, Lacour F
Mol Immunol. 1984 Oct;21(10):939-44. doi: 10.1016/0161-5890(84)90150-0.
A monoclonal antibody to the double-stranded polyribonucleotide complex poly(A) . poly(U) was derived from the fusion of spleen cells from immunized DBA/2 mice and the P3 X X63-Ag8 plasma cytoma. Specificity studies using radioimmunoassays showed that the anti-poly(A) . poly(U) does not cross-react with single-stranded polyribonucleotides. RNA X DNA hybrids or DNAs. In addition to RNA duplexes associating adenine and uracil, it recognizes synthetic poly(I) . poly(C) and naturally occurring reovirus RNA. It is thus directed against a conformational epitope with an absolute requirement for two polyribose phosphate chains. However, the antibody does not cross-react with poly(G) . poly(C) and is therefore able to distinguish between RNA double helices.
一种针对双链多聚核糖核苷酸复合物聚（A）·聚（U）的单克隆抗体，是由免疫后的DBA/2小鼠的脾细胞与P3 X X63-Ag8浆细胞瘤融合产生的。使用放射免疫测定法进行的特异性研究表明，抗聚（A）·聚（U）与单链多聚核糖核苷酸、RNA-DNA杂交体或DNA不发生交叉反应。除了与腺嘌呤和尿嘧啶相关联的RNA双链体外，它还能识别合成的聚（I）·聚（C）和天然存在的呼肠孤病毒RNA。因此，它针对的是一种构象表位，对两条多核糖磷酸链有绝对要求。然而，该抗体与聚（G）·聚（C）不发生交叉反应，因此能够区分RNA双螺旋结构。
