Mikuz G, Hofstaedter F, Delgado R
Anal Quant Cytol Histol. 1985 Dec;7(4):343-6.
A simple technique is presented for the isolation of cells from paraffin-embedded tissues for Feulgen DNA cytophotometric investigations. Tissue fragments from paraffin blocks were deparaffinized in xylene, rehydrated and refixed in a formalin solution and incubated in a solution of 0.5 pepsin in 0.25% hydrochloric acid. After filtration through a 70 micron mesh and centrifugation, the cells were smeared upon a glass slide. Comparison between the results obtained with freshly prepared imprints and with pepsin-extracted cells of the same tumor showed that the extraction technique does not influence the Feulgen reaction or the DNA distribution pattern. Investigations carried out on bladder and embryonal carcinomas have demonstrated that the method permits an analysis of histologically or histochemically identified tumor cells within individual tissue areas.
本文介绍了一种从石蜡包埋组织中分离细胞用于福尔根DNA细胞光度学研究的简单技术。将石蜡块中的组织碎片在二甲苯中脱蜡，复水后在福尔马林溶液中重新固定，然后在含0.5%胃蛋白酶的0.25%盐酸溶液中孵育。经70微米筛网过滤和离心后，将细胞涂片于载玻片上。对同一肿瘤新鲜制备的印片和经胃蛋白酶提取的细胞所获结果进行比较表明，提取技术不影响福尔根反应或DNA分布模式。对膀胱癌和胚胎癌的研究表明，该方法可对单个组织区域内经组织学或组织化学鉴定的肿瘤细胞进行分析。
