Chronic vascular catheterization in the rat: comparison of three techniques.

Rats were implanted with an indwelling vascular cannula in the jugular vein, femoral artery or carotid artery, and evaluated for postsurgical weight changes and cannula patency. Complete details for surgical methods and materials are presented for each procedure. Over a 14 day period, the carotid artery procedure produced the most profound weight loss, while the jugular vein implantation was followed by minimal changes in body weight. Weight loss was intermediate for the femoral artery group. Body weight had returned to, or was above presurgical weight at 2, 4 and 6 days postsurgery for the jugular, femoral and carotid catheterizations, respectively. By 14 days following implantation 83%, 67% and 50% of the femoral, carotid and jugular cannulas, respectively, were patent. We conclude that for long-term sampling of blood in the rat, the femoral artery catheterization procedure is preferable in terms of patency and postsurgical weight loss.