外周血单核白细胞对酵母细胞的吞噬作用：底物的意义。

Yeast cell phagocytosis by mononuclear leucocytes from peripheral blood: significance of the substrate.

作者信息

Rydgren L, Norberg B

出版信息

Scand J Haematol. 1984 Oct;33(4):368-72. doi: 10.1111/j.1600-0609.1984.tb00709.x.

DOI:10.1111/j.1600-0609.1984.tb00709.x

Abstract

Lymphoprep-isolated mononuclear leucocytes (L-MNs) from peripheral blood of healthy donors were mixed with yeast cells and filmed by time-lapse microcinematography in glass chambers at +37 degrees C. The concentration of L-MNs and yeast cells was lowered so as to make directional L-MN movement (chemotaxis) a necessary condition of phagocytosis. 54 cases of phagocytosis were filmed. Only yeast cells lying immobile on the glass surface were engulfed. In addition, 11 collisions between free-floating yeast cells and glass-adherent L-MNs were noted. None of these collisions led to immediate engulfment. It is suggested that the process of phagocytosis requires prolonged leucocyte-prey contact, in the order of magnitude of 3 to 5 seconds.

摘要

从健康供体的外周血中通过淋巴细胞分离液分离得到的单核白细胞（L-MNs）与酵母细胞混合，并于37摄氏度在玻璃小室中通过延时显微电影摄影术进行拍摄。降低L-MNs和酵母细胞的浓度，以使L-MN的定向运动（趋化作用）成为吞噬作用的必要条件。拍摄了54例吞噬作用的情况。只有那些静止在玻璃表面的酵母细胞被吞噬。此外，还观察到11次自由漂浮的酵母细胞与黏附在玻璃上的L-MNs之间的碰撞。这些碰撞均未导致立即吞噬。这表明吞噬过程需要白细胞与猎物长时间接触，时长约为3至5秒。