Harauz G, Ottensmeyer F P
Science. 1984 Nov 23;226(4677):936-40. doi: 10.1126/science.6505674.
Electron spectroscopic imaging was combined with reconstruction algorithms to derive the three-dimensional structure of the nucleosome core particle to a resolution of 1.5 nanometers. Images of phosphorus distributions within individual nucleosomes were interpreted as projections of a supercoil of DNA. These were used to orient the corresponding individual nucleosome images, making it possible to reconstruct the entire nucleosome in three dimensions. The structure is consistent with known biochemical and biophysical data and explains site-specific nuclease sensitivity, although differing in part with other nucleosome models.
电子光谱成像与重建算法相结合，以得出核小体核心颗粒的三维结构，分辨率达到1.5纳米。单个核小体内磷分布的图像被解释为DNA超螺旋的投影。这些投影被用于确定相应单个核小体图像的方向，从而有可能在三维空间中重建整个核小体。该结构与已知的生化和生物物理数据一致，并解释了位点特异性核酸酶敏感性，尽管在部分方面与其他核小体模型不同。
