Schiefer H B, Hancock D S
Toxicol Appl Pharmacol. 1984 Dec;76(3):464-72. doi: 10.1016/0041-008x(84)90350-8.
Topical application of T-2 toxin in dimethyl sulfoxide (DMSO) resulted in the death of 20/20 mice in the 20-, 30-, and 40-mg/kg body weight (BW) dose groups within 4 to 6 days after application, whereas 17/20 and 5/20 animals died in the 10- and 5 mg/kg BW groups, respectively, within 7 days. Histological examination of thymus, spleen, and duodenum at 6, 12, 18, and 24 hr after topical application of 5 or 40 mg/kg BW to mice revealed that the characteristic radiomimetic effects of this trichothecene mycotoxin are easily recognizable at 6 hr after topical application, with the severity of damage being dependent on the organ and time. The lesions are quantitatively and qualitatively identical with those seen after intragastrical application of T-2 toxin. Both the postmortem and, to some extent, the general histological findings were not specific enough to arrive at an etiological diagnosis without prior knowledge of the fact that a mycotoxin was applied to the skin, unless one had the opportunity to look for the characteristic intestinal lesions prior to 24 hr after application, or for necrosis in spleen an thymus after 24 hr.
在二甲基亚砜（DMSO）中局部涂抹T-2毒素，导致体重20、30和40毫克/千克剂量组的20只小鼠在涂抹后4至6天内全部死亡，而体重10毫克/千克和5毫克/千克剂量组分别有17只和5只动物在7天内死亡。对小鼠局部涂抹5或40毫克/千克体重的T-2毒素后6、12、18和24小时的胸腺、脾脏和十二指肠进行组织学检查发现，这种单端孢霉烯族霉菌毒素的典型拟放射性效应在局部涂抹后6小时即可轻易识别，损伤的严重程度取决于器官和时间。这些病变在数量和质量上与经胃内施用T-2毒素后所见的病变相同。除非有机会在施用后24小时内寻找特征性肠道病变，或在24小时后寻找脾脏和胸腺中的坏死，否则在不知道皮肤已施用霉菌毒素这一事实的情况下，尸检结果以及在某种程度上一般的组织学发现都不足以做出病因诊断。
