Rosenbaum A H, Schatzberg A F, MacLaughlin R A, Snyder K, Jiang N S, Ilstrup D, Rothschild A J, Kliman B
Am J Psychiatry. 1984 Dec;141(12):1550-5. doi: 10.1176/ajp.141.12.1550.
he authors used competitive protein binding assay and radioimmunoassay to measure cortisol levels in 38 normal control subjects three times before and three times after administration of 1 mg of dexamethasone. They found significant interassay differences at 11:00 p.m. before dexamethasone and at all three postdexamethasone times. Analysis of variance revealed significant overall positive relationships between age and cortisol levels measured by both techniques. Age correlated significantly with postdexamethasone cortisol levels measured by radioimmunoassay but not when measured by competitive protein binding assay. Clinicians should obtain data from their laboratories as to appropriate cutoffs for cortisol suppression on the specific assay used.
作者采用竞争性蛋白结合分析法和放射免疫分析法，在38名正常对照受试者服用1毫克地塞米松之前三次及之后三次测量皮质醇水平。他们发现在地塞米松给药前晚上11点以及地塞米松给药后的所有三个时间点，不同检测方法之间存在显著差异。方差分析显示，两种检测技术测得的年龄与皮质醇水平之间总体上存在显著的正相关关系。年龄与放射免疫分析法测得的地塞米松给药后皮质醇水平显著相关，但与竞争性蛋白结合分析法测得的结果无关。临床医生应从其实验室获取关于所用特定检测方法中皮质醇抑制的适当临界值的数据。
