Berger P, Kofler R, Wick G
Am J Reprod Immunol (1980). 1984 Jun;5(4):157-60. doi: 10.1111/j.1600-0897.1984.tb00188.x.
Monoclonal antibodies (MCA) against hCG have been characterized with regard to their affinity and their ability to neutralize the biological activity of hCG in vivo. The production and specificities of these reagents were described in the preceding paper of this series. Equilibrium association constants (Ka) of the MCA, determined by radioimmunological saturation assays, ranged from less than 1 X 10(8)M-1 up to 3.7 X 10(9)M-1 whereas values for conventional polyclonal antisera against hCG ranged from 8.9 X 10(9)M-1 to 1.8 X 10(10)M-1. The ability of MCA to neutralize the biological activity of hCG was tested in a rat bioassay in vivo; 9 of 13 different MCA preparations tested could neutralize hCG. Surprisingly, this property did not correlate with affinity or specificity, and was not restricted to those MCA recognizing the hormone specific beta-subunit. It could be demonstrated that determinants on each individual subunit as well as epitopes formed by both subunits are involved in the expression of the biological activity of hCG.
针对人绒毛膜促性腺激素（hCG）的单克隆抗体（MCA）已在亲和力及其在体内中和hCG生物活性的能力方面得到了表征。这些试剂的制备及特异性在本系列的前一篇论文中已有描述。通过放射免疫饱和分析测定的MCA的平衡缔合常数（Ka）范围为小于1×10⁸M⁻¹至3.7×10⁹M⁻¹，而针对hCG的传统多克隆抗血清的值范围为8.9×10⁹M⁻¹至1.8×10¹⁰M⁻¹。在大鼠体内生物测定中测试了MCA中和hCG生物活性的能力；所测试的13种不同MCA制剂中有9种能够中和hCG。令人惊讶的是，这一特性与亲和力或特异性无关，并且不限于那些识别激素特异性β亚基的MCA。可以证明，每个亚基上的决定簇以及由两个亚基形成的表位都参与了hCG生物活性的表达。
