Pichinoty F, de Berjac H, Mandel M, Greenway B, Garcia J L
Ann Microbiol (Paris). 1976 Oct;127B(3):351-61.
The described bacterium was isolated by enrichment culture in peptone broth inoculated with garden soil, pasteurized and then put to incubate under N2O at 32 degrees. It is a Gram-negative rod, motile with peritrichous flagella, and producing oval spores without exosporium in swollen sporangia. However, cells have the thick walls, mesosomes and persistant septa characteristic of Gram-positive bacteria. It lacks fermentative activity, does not attack carbohydrates, has complex growth requirements, and will grow anaerobically only if one of the following electron acceptors is present: NO3, NO2, N2O, S4O6, and fumarate. Nitrate, nitrite, and nitrous oxide are denitrified with production of N2. The microorganism is mesophilic, gives a positive oxidase reaction, synthesizes a type of c cytochrome, and does not hydrolyse gelatin, starch nor "Tween 80". The following enzymes are present: nitrate reductase A, respiratory nitrite reductase, tetrathionate and fumarate reductases, L-glutamate dehydrogenase, and superoxide dismutase. The following enzymes are absent: thiosulfate reductase, urease, lecithinase, arginine dihydrolase, L-alanine dehydrogenase, phenylalanine desaminase, and catalase. The GC% of its DNA is 39. The bacterium described can be considered to be a new species. We propose the name Bacillus azotoformans n. sp.
所述细菌是通过在接种了花园土壤的蛋白胨肉汤中进行富集培养分离得到的，将其巴氏灭菌后，置于32℃的一氧化二氮环境下培养。它是革兰氏阴性杆菌，具周生鞭毛能运动，在膨大的孢子囊中产生无芽孢外壁的椭圆形孢子。然而，细胞具有革兰氏阳性菌特有的厚壁、中体和持久隔膜。它缺乏发酵活性，不分解碳水化合物，生长需求复杂，只有在存在以下电子受体之一时才能厌氧生长：硝酸根、亚硝酸根、一氧化二氮、连四硫酸根和富马酸根。硝酸根、亚硝酸根和一氧化二氮被反硝化产生氮气。该微生物是嗜温菌，氧化酶反应呈阳性，合成一种c型细胞色素，不水解明胶、淀粉和“吐温80”。存在以下酶：硝酸还原酶A、呼吸亚硝酸还原酶、连四硫酸根还原酶和富马酸根还原酶、L-谷氨酸脱氢酶和超氧化物歧化酶。不存在以下酶：硫代硫酸根还原酶、脲酶、卵磷脂酶、精氨酸双水解酶、L-丙氨酸脱氢酶、苯丙氨酸脱氨酶和过氧化氢酶。其DNA的鸟嘌呤和胞嘧啶含量为39%。所描述的细菌可被认为是一个新物种。我们提议将其命名为固氮芽孢杆菌，新种。
