Bilinski C A, Sills A M, Stewart G G
Appl Environ Microbiol. 1984 Oct;48(4):813-7. doi: 10.1128/aem.48.4.813-817.1984.
An enrichment procedure after ethyl methanesulfonate mutagenesis and exposure to the fungicide benomyl yielded mutants auxotrophic for several amino acids from two polyploid Saccharomyces spp. Benomyl treatment was found to have a marked morphological effect on polyploid Saccharomyces cerevisiae, causing cells to adopt a characteristic doublet cell morphology in which buds are nearly as large as the parent cells. Experiments in which nuclear division was monitored in benomyl-induced doublet cells by Giemsa nuclear staining demonstrated an unusual sequence of cytological events which culminated in the formation of binucleate parental and mononucleate bud components. The frequency of formation of doublet and binucleate parent cells was found to depend on the strain employed and the benomyl concentration administered.
甲磺酸乙酯诱变并接触杀菌剂苯菌灵后进行的富集程序，从两个多倍体酿酒酵母属物种中获得了几种氨基酸营养缺陷型突变体。发现苯菌灵处理对多倍体酿酒酵母有显著的形态学影响，导致细胞呈现出特征性的双细胞形态，其中芽几乎与母细胞一样大。通过吉姆萨核染色在苯菌灵诱导的双细胞中监测核分裂的实验表明，细胞事件的顺序异常，最终形成双核母细胞和单核芽组分。发现双细胞和双核母细胞的形成频率取决于所用菌株和苯菌灵施用浓度。
