Quinlan R A, Pogson C I, Gull K
J Cell Sci. 1980 Dec;46:341-52. doi: 10.1242/jcs.46.1.341.
Methyl benzimidazol-2-yl-carbamate (MBC), at a concentration of 100 microM, has a pronounced effect on the growth of Saccharomyces cerevisiae, resulting in the accumulation of cells as large doublets. We have determined a specific execution point for the effect of MBC on the yeast cell cycle, and have shown that this execution point is between the cycle events of spindle pole body duplication and spindle pole body separation. An ultrastructural examination of the MBC-treated cells revealed the absence of cytoplasmic and spindle microtubules. MBC treatment also produced an altered spindle pole body morphology, causing the disappearance of the outer component. Nuclear size was also markedly increased in the MBC-induced doublet cells, although the septa were completely absent from these doublet cells. It is proposed that MBC inhibits microtubule polymerization, rather than causing the depolymerization of stable microtubules.
浓度为100微摩尔的甲基苯并咪唑-2-基氨基甲酸酯（MBC）对酿酒酵母的生长有显著影响，导致细胞积累形成大的双核体。我们已经确定了MBC对酵母细胞周期影响的一个特定执行点，并表明该执行点位于纺锤极体复制和纺锤极体分离的细胞周期事件之间。对经MBC处理的细胞进行超微结构检查发现，细胞质微管和纺锤体微管均不存在。MBC处理还导致纺锤极体形态改变，致使外部成分消失。在MBC诱导形成的双核体细胞中，细胞核大小也显著增加，尽管这些双核体细胞中完全没有隔膜。有人提出，MBC抑制微管聚合，而不是导致稳定微管解聚。
