Kren V, Pazoutová S, Rylko V, Sajdl P, Wurst M, Rehácek Z
Appl Environ Microbiol. 1984 Oct;48(4):826-9. doi: 10.1128/aem.48.4.826-829.1984.
Transformation of extracellular sucrose during cultivation of Claviceps purpurea led to the formation of mono- and oligosaccharides. Maltose was a suitable substrate for submerged fermentation of alkaloids. Fermentation in a medium with maltose was characterized by an insignificant formation of glucans, intensive sporulation, suspension growth of mycelium, and a higher formation of elymoclavine. Glucose alone yielded low levels of total alkaloids and high glucan formation; on the other hand, glucose promoted the formation of elymoclavine.
在紫麦角菌培养过程中,胞外蔗糖的转化导致了单糖和寡糖的形成。麦芽糖是生物碱深层发酵的合适底物。在含有麦芽糖的培养基中发酵的特征是葡聚糖形成不显著、孢子大量形成、菌丝体悬浮生长以及麦角新碱形成较多。单独使用葡萄糖时,总生物碱产量较低且葡聚糖形成较多;另一方面,葡萄糖促进了麦角新碱的形成。