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Extracellular metabolism of sucrose in a submerged culture of Claviceps purpurea: formation of monosaccharides and clavine alkaloids.麦角菌深层培养中蔗糖的胞外代谢：单糖和棒麦角碱的形成
Appl Environ Microbiol. 1984 Oct;48(4):826-9. doi: 10.1128/aem.48.4.826-829.1984.
2
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4
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J Gen Microbiol. 1974 Jun;82(2):345-61. doi: 10.1099/00221287-82-2-345.
5
Parasitic fungus Claviceps as a source for biotechnological production of ergot alkaloids.寄生于真菌麦角菌 Claviceps 可作为生物科技生产麦角生物碱的原料。
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6
Comparison of ergot alkaloid biosynthesis gene clusters in Claviceps species indicates loss of late pathway steps in evolution of C. fusiformis.麦角菌属物种中麦角生物碱生物合成基因簇的比较表明，梭形麦角菌在进化过程中后期途径步骤有所缺失。
Appl Environ Microbiol. 2007 Nov;73(22):7185-91. doi: 10.1128/AEM.01040-07. Epub 2007 Aug 24.
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The cellular role of nitrogen in the biosynthesis of alkaloids by submerged culture of Claviceps purpurea (Fr.) Tul.氮在紫麦角菌（Claviceps purpurea (Fr.) Tul.）深层培养生物碱生物合成中的细胞作用
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Induction of sclerotialike mycelium in axenic cultures of Claviceps purpurea producing clavine alkaloids.在产生棒麦角生物碱的麦角菌无菌培养物中诱导类菌核菌丝体。
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9
Production of alkaloids and differentiation in a submerged culture of Claviceps purpurea (Fr.) Tul.麦角菌（Claviceps purpurea (Fr.) Tul.）深层培养中生物碱的产生与分化
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10
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Saudi J Biol Sci. 2018 Mar;25(3):529-536. doi: 10.1016/j.sjbs.2016.01.020. Epub 2016 Feb 13.
2
Saprophytic production of clavine alkaloids and activity of hydroxymethylglutaryl-CoA reductase.棒麦角生物碱的腐生生产及羟甲基戊二酰辅酶A还原酶的活性
Folia Microbiol (Praha). 1986;31(4):282-7. doi: 10.1007/BF02926952.
3
Formation of cyclosporins by Tolypocladium inflatum.
Folia Microbiol (Praha). 1991;36(6):549-56. doi: 10.1007/BF02884035.
4
Physiological controls and regulation of ergot alkaloid formation.麦角生物碱形成的生理控制与调节
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本文引用的文献

1
Beta-D-1, 3 Glucanases in fungi.真菌中的β-D-1,3-葡聚糖酶
Can J Microbiol. 1959 Apr;5(2):173-85. doi: 10.1139/m59-022.
2
Transfer reactions catalysed by some sucrase preparations.某些蔗糖酶制剂催化的转移反应。
Biochem J. 1954 May;57(1):22-33. doi: 10.1042/bj0570022.
3
Gas chromatographic determination of extracellular metabolites produced by baker's yeast during glucose-induced acidification.气相色谱法测定面包酵母在葡萄糖诱导酸化过程中产生的细胞外代谢物。
Folia Microbiol (Praha). 1980;25(4):306-10. doi: 10.1007/BF02876610.
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Formation and structure of extracellular glucans produced by Claviceps species.麦角菌属物种产生的细胞外葡聚糖的形成与结构
J Gen Microbiol. 1968 May;51(3):337-52. doi: 10.1099/00221287-51-3-337.
5
Production of clavine-alkaloids by Claviceps fusiformis (Loveless) in submerged culture.梭形麦角菌（洛夫莱斯）在深层培养中产生棒麦角生物碱。
Acta Microbiol Acad Sci Hung. 1968;15(1):29-40.
6
Studies on sucrose breakdown by invertase-producing Claviceps strains.
Acta Microbiol Acad Sci Hung. 1968;15(1):17-28.
7
Autolysis of extracellular glucans produced in vitro by a strain of Claviceps fusiformis.
J Gen Microbiol. 1970 Mar;60(3):403-15. doi: 10.1099/00221287-60-3-403.
8
Ergotamine production and metabolism of Claviceps purpurea strain 275 FI in stirred fermenters.
Can J Microbiol. 1970 Oct;16(10):923-31. doi: 10.1139/m70-159.
9
Physiological studies on ergot: further studies on the induction of alkaloid synthesis by tryptophan and its inhibition by phosphate.麦角的生理学研究：关于色氨酸诱导生物碱合成及其受磷酸盐抑制的进一步研究。
J Bacteriol. 1972 Nov;112(2):791-6. doi: 10.1128/jb.112.2.791-796.1972.
10
Comparative metabolism of Claviceps purpurea in vivo and in vitro.麦角菌在体内和体外的比较代谢
Biochem J. 1972 Apr;127(2):3P-4P. doi: 10.1042/bj1270003p.

麦角菌深层培养中蔗糖的胞外代谢：单糖和棒麦角碱的形成

Extracellular metabolism of sucrose in a submerged culture of Claviceps purpurea: formation of monosaccharides and clavine alkaloids.

作者信息

Kren V, Pazoutová S, Rylko V, Sajdl P, Wurst M, Rehácek Z

出版信息

Appl Environ Microbiol. 1984 Oct;48(4):826-9. doi: 10.1128/aem.48.4.826-829.1984.

DOI:10.1128/aem.48.4.826-829.1984
PMID:6508291
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC241621/
Abstract

Transformation of extracellular sucrose during cultivation of Claviceps purpurea led to the formation of mono- and oligosaccharides. Maltose was a suitable substrate for submerged fermentation of alkaloids. Fermentation in a medium with maltose was characterized by an insignificant formation of glucans, intensive sporulation, suspension growth of mycelium, and a higher formation of elymoclavine. Glucose alone yielded low levels of total alkaloids and high glucan formation; on the other hand, glucose promoted the formation of elymoclavine.

摘要

在紫麦角菌培养过程中，胞外蔗糖的转化导致了单糖和寡糖的形成。麦芽糖是生物碱深层发酵的合适底物。在含有麦芽糖的培养基中发酵的特征是葡聚糖形成不显著、孢子大量形成、菌丝体悬浮生长以及麦角新碱形成较多。单独使用葡萄糖时，总生物碱产量较低且葡聚糖形成较多；另一方面，葡萄糖促进了麦角新碱的形成。