Patsch W, Patsch J R, Kostner G M, Sailer S, Braunsteiner H
J Biol Chem. 1978 Jul 25;253(14):4911-5.
Very low density lipoproteins (VLDL) have been isolated and subfractionated on the basis of their differing flotation rates. The procedure consists of a single 45-min zonal ultracentrifugation step using a linear density gradient of d = 1.00 to 1.15 g/ml. Appropriate fractions of the zonal rotor effluent containing the entire VLDL spectrum were characterized by analytical ultracentrifugation, gel filtration chromatography, and complete chemical analysis. Flotation rates of VLDL subspecies from hypertriglyceridemic and normolipemic plasmas correlated directly with their Stokes radii and triglyceride content and inversely with their proportion of cholesterol, cholesteryl esters, phospholipids, and total protein. There was also an inverse correlation of flotation rate with the fraction of tetramethylurea-insoluble protein. This procedure provides a reliable methodology for a rapid isolation of VLDL subfractions and the accurate determination of their flotation rates.
极低密度脂蛋白（VLDL）已根据其不同的漂浮率进行分离和亚分级。该程序包括使用d = 1.00至1.15 g/ml的线性密度梯度进行单次45分钟的区带超速离心步骤。通过分析超速离心、凝胶过滤色谱和完整的化学分析对包含整个VLDL谱的区带转子流出物的适当部分进行表征。来自高甘油三酯血症和正常血脂血浆的VLDL亚类的漂浮率与其斯托克斯半径和甘油三酯含量直接相关，与胆固醇、胆固醇酯、磷脂和总蛋白的比例成反比。漂浮率与四甲基脲不溶性蛋白的比例也呈负相关。该程序为快速分离VLDL亚组分和准确测定其漂浮率提供了一种可靠的方法。
