通过低水平化学发光检测α-生育酚在微粒体脂质过氧化中的抗氧化活性评估。
Evaluation of alpha-tocopherol antioxidant activity in microsomal lipid peroxidation as detected by low-level chemiluminescence.
作者信息
Cadenas E, Ginsberg M, Rabe U, Sies H
出版信息
Biochem J. 1984 Nov 1;223(3):755-9. doi: 10.1042/bj2230755.
The significance of microsomal vitamin E in protecting against the free-radical process of lipid peroxidation was evaluated with the low-level-chemiluminescence technique in microsomal fractions from vitamin E-deficient and control rats. The induction period that normally precedes the ascorbate/ADP/Fe3+-induced lipid peroxidation was taken as reflecting the microsomal vitamin E content and was found to be 5-6-fold decreased in microsomal fractions from vitamin E-deficient rats. Supplementation of microsomal fractions from vitamin E-deficient rats with exogenous vitamin E partially restores the induction period observed in that from control rats. The decrease in chemiluminescence intensity and the increase in the induction period both correlate linearly with the amount of vitamin E added. However, the efficiency of exogenous vitamin E is about 50-fold lower than that exerted by the naturally occurring vitamin E in microsomal membranes. These observations are discussed in terms of the process of re-incorporation of vitamin E into membranes, the experimental model for lipid peroxidation selected, and the method to evaluate lipid peroxidation, namely low-level chemiluminescence.
采用低水平化学发光技术，在维生素E缺乏和对照大鼠的微粒体组分中，评估微粒体维生素E在防止脂质过氧化自由基过程中的意义。将抗坏血酸/ADP/Fe3+诱导的脂质过氧化之前的正常诱导期作为反映微粒体维生素E含量的指标，发现维生素E缺乏大鼠的微粒体组分中诱导期降低了5-6倍。用外源性维生素E补充维生素E缺乏大鼠的微粒体组分，可部分恢复在对照大鼠微粒体中观察到的诱导期。化学发光强度的降低和诱导期的增加均与添加的维生素E量呈线性相关。然而，外源性维生素E的效率比微粒体膜中天然存在的维生素E低约50倍。根据维生素E重新掺入膜的过程、选择的脂质过氧化实验模型以及评估脂质过氧化的方法（即低水平化学发光）对这些观察结果进行了讨论。
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