Deguchi T, Okumura S, Shimizu M
Jpn J Antibiot. 1978 Mar;31(3):149-52.
A radioimmunoassay for an aminoglycoside antibiotic, sagamicin, was developed using antisera from rabbits injected with a sagamicin-bovine serum albumin conjugate. Sagamicin was iodinated by a modified Bolton & Hunter method. The standard curve was linear on a logit-log plot yielding an sensitivity of 0.5ng/tube. A correlation coefficient of 0.95 was obtained between the radioimmunoassay and a microbioassay for sagamicin in human sera. Cross-reaction occurred with gentamicin components and some of sagamicin subunits, but there was no cross-reactivity to neomycin, kanamycin and amikacin. Antibody was purified by affinity chromatography on sagamicin bound agarose.
利用注射了紫苏霉素-牛血清白蛋白偶联物的兔血清，开发了一种用于氨基糖苷类抗生素紫苏霉素的放射免疫测定法。紫苏霉素通过改良的博尔顿-亨特法进行碘化。标准曲线在logit-log图上呈线性，灵敏度为0.5ng/管。放射免疫测定法与人类血清中紫苏霉素的微生物测定法之间的相关系数为0.95。庆大霉素组分和一些紫苏霉素亚基会发生交叉反应，但与新霉素、卡那霉素和阿米卡星无交叉反应。通过在结合了紫苏霉素的琼脂糖上进行亲和层析来纯化抗体。
