Gelosa L, Manera A
Boll Ist Sieroter Milan. 1984 Jul 31;63(3):254-61.
168 serum specimens have been tested for Leptospirosis in the years from 1980 to 1983. The identification procedure was a macroscopic plate agglutination screening test with formalin fied antigens and a confirmatory microscopic agglutination-lysis test of live antigens of actively growing cultures of different pathogenic serotypes propagated in a fluid medium. 34 serum specimens (23.8%) relevant to 18 cases were positive. L. icterohaemorrhagiae was positive in 12 cases (63.1%), L. pomona in 2 cases (10.5%), L. canicola in 1 case (5.3%), 1 case was positive for both L. grippotyphosa and L. bataviae and 1 case was positive for L. poi and L. sejroe. During the summer-fall of 1981 40 specimens of surface water from 4 pre-alpin lakes of the Provincia of Como were tested for Leptospiras with the cultural and biological examinations, following the detection of several leptospirosis cases on the relevant area. All water specimens gave negative results.
1980年至1983年期间，对168份血清样本进行了钩端螺旋体病检测。鉴定程序包括使用福尔马林固定抗原的宏观平板凝集筛选试验，以及对在液体培养基中繁殖的不同致病血清型的活跃生长培养物的活抗原进行确证性显微镜凝集溶解试验。与18例病例相关的34份血清样本（23.8%）呈阳性。出血性黄疸钩端螺旋体阳性12例（63.1%），波摩那钩端螺旋体阳性2例（10.5%），犬钩端螺旋体阳性1例（5.3%），1例犬型钩端螺旋体和巴达维亚钩端螺旋体均呈阳性，1例波蒙那钩端螺旋体和赛罗群钩端螺旋体呈阳性。1981年夏秋季节，在科莫省4个阿尔卑斯山前湖泊采集了40份地表水样本，在相关区域检测到几例钩端螺旋体病病例后，对这些样本进行了培养和生物学检查以检测钩端螺旋体。所有水样检测结果均为阴性。
