Cacciapuoti B
Boll Ist Sieroter Milan. 1984 Jul 31;63(3):237-43.
Confirmatory diagnosis of clinical leptospirosis may be achieved by each hospital laboratory performing all-purpose bacteriological methods. Direct microscopic examination of blood may frequently demonstrate circulating leptospiras in the first few days of pyrexia. Leptospiras may be tentatively cultured from blood in the same initial period of illness, in fluid semisynthetic commercial media according to the original Ellinghausen medium. Sero-conversion for leptospira antibodies is constantly observed on two serum samples taken in the first few days of illness and ten days thereafter respectively, in each case of current leptospirosis.
各医院实验室采用通用细菌学方法可实现临床钩端螺旋体病的确证诊断。发热初期的几天内，对血液进行直接显微镜检查常常可发现循环中的钩端螺旋体。在疾病初期的同一阶段，可尝试将血液接种于根据原始埃林豪森培养基改良的半合成商业液体培养基中进行钩端螺旋体培养。在每例现症钩端螺旋体病病例中，分别于发病初期的几天内和之后十天采集的两份血清样本中，均可持续观察到钩端螺旋体抗体的血清学转化。
