Shirahama T, Skinner M, Cohen A S
Cell Biol Int Rep. 1984 Oct;8(10):849-56. doi: 10.1016/0309-1651(84)90068-7.
Amyloid protein AA is believed to be synthesized in the liver in a form of its precursor, the acute phase reactant, SAA. To identify precisely the cell involved in SAA biosynthesis, immunohistochemical reaction to anti-mouse AA was analysed on the liver sections from CBA/J mice which had received a single casein injection and were sacrificed at predetermined times up to 48 hours. Two different primary localizations of the reaction were revealed. One was in the cytoplasm of certain hepatocytes. This reaction peaked at 6-8 hours after the casein injection in the intensity of the reaction and in the number of the cells involved. The hepatocytes with positive cytoplasmic reaction were scattered over the lobule and even at the peak did not exceed 20% of the total hepatocyte population. The other localization of the reaction covered linearly the surface of the hepatocyte cords, and peaked at 12-16 hours after the casein injection.
淀粉样蛋白AA被认为是以其前体即急性期反应物血清淀粉样蛋白A（SAA）的形式在肝脏中合成的。为了精确鉴定参与SAA生物合成的细胞，对接受单次酪蛋白注射并在长达48小时的预定时间处死的CBA/J小鼠肝脏切片进行了抗小鼠AA的免疫组织化学反应分析。发现了反应的两种不同主要定位。一种位于某些肝细胞的细胞质中。这种反应在酪蛋白注射后6 - 8小时在反应强度和涉及的细胞数量上达到峰值。细胞质反应阳性的肝细胞散布在小叶中，即使在峰值时也不超过肝细胞总数的20%。反应的另一定位呈线性覆盖肝细胞索表面，并在酪蛋白注射后12 - 16小时达到峰值。
