Palm M, Nielsen E H, Svehag S E
Department of Medical Microbiology, Odense University, Denmark.
APMIS. 1997 Aug;105(8):603-8. doi: 10.1111/j.1699-0463.1997.tb05059.x.
Different conditions for establishing a cell culture system for generation of AA-amyloid were investigated. The most effective system was based on peritoneal macrophages from CBA/J mice that had received repeated injections of Hammersten casein, with subsequent cultivation of the cells at high density, high levels of acute phase serum, and neutral pH. Staining with Congo red, thioflavin T, and anti-AA revealed amyloid-like structures associated with macrophage clusters. The structures increased in number and size from day 2 to 6 of cell cultivation. The concentration of apoSAA in the culture medium fell markedly in the amyloid-producing cell cultures, while the SAP concentration was not reduced. The described cell culture system can be useful in studies of the influence of chaperone molecules and other factors or the formation and degradation of amyloid fibrils.
研究了建立用于生成AA淀粉样蛋白的细胞培养系统的不同条件。最有效的系统基于来自CBA/J小鼠的腹腔巨噬细胞，这些小鼠接受了反复注射Hammersten酪蛋白，随后在高密度、高水平急性期血清和中性pH条件下培养细胞。用刚果红、硫黄素T和抗AA染色显示与巨噬细胞簇相关的淀粉样样结构。从细胞培养的第2天到第6天，这些结构的数量和大小增加。在产生淀粉样蛋白的细胞培养物中，培养基中载脂蛋白SAA的浓度显著下降，而血清淀粉样蛋白P成分（SAP）的浓度没有降低。所描述的细胞培养系统可用于研究伴侣分子和其他因素对淀粉样纤维形成和降解的影响。
