Mothersill C, Seymour C B
Cell Biol Int Rep. 1984 Oct;8(10):887-96. doi: 10.1016/0309-1651(84)90073-0.
A method has been developed which allows four stages of transformation to be detected in subcultured populations of sheep thyroid cells exposed to gamma irradiation as differentiated primary cultures. The cell populations initially irradiated and the subcultures deriving from these retain a number of differentiated properties of thyroid cells, including iodine uptake, follicle formation and T4 production. The stages of transformation which develop in the subcultures are nonsenescence, focus development on a confluent monolayer, i.e. loss of contact inhibition, colony formation in soft agar, i.e. anchorage independence, and reduced serum dependence. The technique offers a method for detecting the development of transformation in primary differentiated epithelial cultures.
已开发出一种方法，该方法可在作为分化原代培养物暴露于γ射线照射的绵羊甲状腺细胞亚培养群体中检测到四个转化阶段。最初接受照射的细胞群体以及从这些群体衍生而来的亚培养物保留了甲状腺细胞的许多分化特性，包括碘摄取、滤泡形成和T4产生。在亚培养物中发展的转化阶段为非衰老、在汇合单层上形成集落，即失去接触抑制、在软琼脂中形成菌落，即不依赖贴壁，以及降低血清依赖性。该技术提供了一种在原代分化上皮培养物中检测转化发展的方法。
