Veith M, Taylor D W, Thorne K, Richardson B A, Butterworth A E
Clin Exp Immunol. 1984 Dec;58(3):603-10.
Previous studies have shown that human mononuclear cells produce during culture an activity that enhances the functional properties of mature eosinophils, including their helminthotoxic potential (Veith & Butterworth, 1983). We have now found that the enhancing activity in mononuclear cell culture supernatants (MCS) is stable to dialysis for 24 h at pH 2 and sensitive to trypsin (0.1 mg/ml). It has a principal pI of 4.4 and possible secondary peaks with pI values of 5.0 and 5.7. The effect of MCS on eosinophils appears to be a general early stimulation of several functions. In comparison with eosinophils incubated in medium only, the proportion of eosinophils with Fc receptors is increased after incubation with MCS for 3.5 h (39 +/- 11% vs 16 +/- 3%) and 7 h (28 +/- 4% vs 17 +/- 3%). The temperature-dependent phase of eosinophil adherence to antibody coated schistosomula is enhanced after 3-4 h. The uptake of deoxyglucose is enhanced after a 1 h pre-incubation with MCS (24 +/- 1 X 10(3) d/min), compared with that of controls (15 +/- 2 X 10(3) d/min). Whether these effects are due to one mediator in MCS awaits further investigation.
先前的研究表明，人单核细胞在培养过程中会产生一种活性物质，该物质可增强成熟嗜酸性粒细胞的功能特性，包括其对蠕虫的毒性潜力（Veith和Butterworth，1983年）。我们现已发现，单核细胞培养上清液（MCS）中的增强活性在pH 2条件下透析24小时后仍保持稳定，且对胰蛋白酶（0.1 mg/ml）敏感。其主要的等电点为4.4，可能还有等电点为5.0和5.7的次要峰。MCS对嗜酸性粒细胞的作用似乎是对多种功能的一般性早期刺激。与仅在培养基中孵育的嗜酸性粒细胞相比，用MCS孵育3.5小时（39±11%对16±3%）和7小时（28±4%对17±3%）后，具有Fc受体的嗜酸性粒细胞比例增加。嗜酸性粒细胞对抗体包被的血吸虫幼虫的温度依赖性黏附阶段在3 - 4小时后增强。与对照组（15±2×10³ d/min）相比，用MCS预孵育1小时后，脱氧葡萄糖的摄取增强（24±1×10³ d/min）。这些作用是否归因于MCS中的一种介质有待进一步研究。
